| 吉西他滨白蛋白纳米粒对胰腺癌细胞株SW1990体外增殖抑制效应的实验研究 |
| |
| 引用本文： | 陈润浩,李金明,徐近,崔龙,金忱,傅德良,倪泉兴. 吉西他滨白蛋白纳米粒对胰腺癌细胞株SW1990体外增殖抑制效应的实验研究[J]. 中国临床医学, 2009, 16(5): 728-730 |
| |
| 作者姓名： | 陈润浩 李金明 徐近 崔龙 金忱 傅德良 倪泉兴 |
| |
| 作者单位： | 1. 复旦大学附属华山医院普外科,复旦大学胰腺病研究所,上海,200040
2. 上海交通大学医学院附属新华医院肛肠外科,上海,200092 |
| |
| 基金项目： | 上海市经委项目(07ZH-028);;上海市科委项目(08431902500) |
| |
| 摘 要： | 目的：通过体外实验,探讨吉西他滨（GEM）白蛋白纳米粒对人胰腺癌细胞株SW1990体外增殖的抑制效应。方法：以人胰腺癌细胞株SW1990为研究对象,分为5个给药组：110nm GEM纳米粒组、406nm GEM纳米粒组、单纯GEM组、空白纳米粒组和无药对照组;运用MTT法检测给药后48h和72h的增殖抑制率。结果：MTT试验检测提示110nm GEM-NP,406nm-GEM-NP和GEM均具有显著的细胞抑制作用,且72h后406nm-GEM-NP的细胞抑制率在50μg·mL-1GEM浓度时,超过了单纯GEM（P〈0.05）,表明其有显著的药物缓释作用;空白纳米粒对细胞抑制轻微,且不具有时间和浓度依赖性。结论：GEM白蛋白纳米粒,特别是406nm-GEM-NP,对人胰腺癌细胞株SW1990具有显著的体外细胞增殖抑制作用,并且生物相容性良好。
|
| 关 键 词： | 吉西他滨 纳米粒 白蛋白 胰腺癌 SW1990 |
Cytotoxicity of Gemcitabine-Loaded Albumin Nanoparticles on Human Pancreatic Cancer Cells SW1990 in Vitro |
| |
| CHEN Runhao,LI Jinming,XU Jing,CUI Long,JIN Chen,FU Deliang,NI Quanxing. Cytotoxicity of Gemcitabine-Loaded Albumin Nanoparticles on Human Pancreatic Cancer Cells SW1990 in Vitro[J]. Chinese Journal Of Clinical Medicine, 2009, 16(5): 728-730 |
| |
| Authors: | CHEN Runhao LI Jinming XU Jing CUI Long JIN Chen FU Deliang NI Quanxing |
| |
| Affiliation: | CHEN Runhao1 LI Jinming2 XU Jing1 CUI Long2 JIN Chen1 FU Deliang1 NI Quanxing1 1. Pancreatic Disease Institute,Department of General Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China 2. Department of Colorectal & Anal Surgery,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200092,China |
| |
| Abstract: | Objective:To investigate the anti-proliferation effect of gemcitabine-loaded albumin nanoparticles on human pancreatic cancer cells,SW1990,in vitro. Methods: SW1990 cells were treated with 110nm GEM-loaded albumin nanoparticles,406nm GEM-loaded albumin nanoparticles,pure GEM,blank albumin nanoparticles and culture fluid respectively. SW1990 cell growth inhibition rates were detected after being treated with drug gradient by MTT essay,and also detected at 48h and 72h after administration. Results: 110nm GEM-... |
| |
| Keywords: | Gemcitabine Nanoparticle Albumin Pancreatic cancer SW1990 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
