细胞色素c在再灌注肺损伤中的作用及葛根素对其的干预

目的：探讨细胞色素c（cyt-c）在肺缺血再灌注损伤中的作用及葛根素对其的影响。方法：采用大鼠单肺缺血再灌注损伤模型。30只wistar大鼠,随机分为三组：对照组（C组）、缺血再灌注组（IR组）和葛根素干预组（Pur组）。采用原位缺口末端标记（TUNEL）法检测肺组织细胞凋亡,免疫组化SP法检测cyt-c和caspase-3蛋白的表达。结果：①IR组肺组织细胞cyt-c和caspase-3蛋白表达较C组明显增强,细胞凋亡指数显著升高（P〈0.01）;葛根素干预后,cyt-c、caspase-3及细胞凋亡指数均明显下调（P〈0.01）。②cyt-c表达水平与caspase-3和细胞凋亡指数均呈显著正相关（P〈0.01）。结论：cyt-c参与肺缺血再灌注损伤的病理过程,葛根素可能通过抑制cyt-c通路保护肺组织。

Effect of cytochrome c in lung ischemia reperfusion injury and antagonism of puerarin on it

Objective：To explore the role of cytochrome c（cyt-c） in lung ischemia reperfusion injury and antagonism of puerarin on it.Methods：Rat lung model of ischemia reperfusion injury was established in vivo.Thirty Wistar rats were divided into three groups randomly：control group（group C）,ischemia reperfusion group（group IR） and puerarin group（group Pur）.The phenomenon of pulmonary apoptosis with TUNEL and the expression of cyt-c and caspase-3 with immunohistochemistry SP were studied.Results：①Expression of cyt-c and caspase-3 as well as AI in group IR were increased evidently compared with group C（P〈0.01）;while the same changes happened in group Pur but less severely（P〈0.01）.②The relation analysis indicated that the expression of cyt-c was strongly positively correlative to caspase-3 and apoptosis index（AI）（P〈0.01）.Conclusion：Overexpression of cyt-c after lung ischemia reperfusion may play a significant role in the pathogenesis of lung injury,and puerarin may protect lung tissue by down-regulating expression of cyt-c.