硒对胃癌大鼠肾上腺皮质3β-HSD和SDH组织化学的影响

目的：观察硒在预防大鼠实验性胃粘膜癌变过程中肾上腺皮质组织化学的变化。方法：用断乳雄性Wistar大鼠，分为盐水对照组、N-甲基N—硝基—亚硝基胍(MNNG)和Selenite十MNNG组，用组织化学和图像分析方法观察大鼠肾上腺皮质的变化。结果：补硒组大鼠肾上腺皮质束状带和网状带细胞内琥珀酸脱氢酶(SDH)和3β—羟基类固醇脱氢酶(3β—HSD)的组化反应减弱，与MNNG组相比有显著性差异，与对照组相比略有增加，但无统计学意义。MNNG组大鼠SDH和3β—HSD的反应增强，与对照组相比有显著性差异。结论：硒在预防大鼠实验性胃癌过程中，可能参与了肾上腺皮质功能的调节。

Histochemical effects of selenium on adrenal cortex in experimental rats with gastric cancer

Objective:To observe histochemical changes produced by selenium(Se) on the adrenal cortex in experimental rats with gastric cancer. Methods: Weanling Wistar male rats were divided into saline control groups,N-methyl-N-nitro-N-nitrosoguanidine (MNNG) group,Se supplementation group before MNNG gavage. The changes of the adrenal cortex were studied by histochemistry and image analysis. Results:Compared with MNNG group,the cells of fascicular and retic-ular zones of adrenal cortex in Se group showed decreased activity of sucinic dehydrogenase (SDH) and 3-hydroxysteriold dyhydrogense (3-HSD),but the difference in comparison with control group was insignificant. The activity of 3-HSD and SDH in MNNG group increased significantly compared with control group. Conclusion: The results indicate that Se might participate in the regulation of adrenal cortex function.