不同治疗方法对急性重症胆管炎大鼠肺损伤的影响

探讨不同的治疗方法对急性重症胆管炎（ACST）并发肺损伤的影响及治疗机制。采用(35)~S标记大肠杆菌为示踪物观察肠道细菌易位；(125)~I标记的牛血清白蛋白测定肺微血管通透性（PMP）；电子自旋共振仪测定组织中氧自由基（OFR）含量；生化方法测定组织中磷脂酶A_2（PLA_2）活性。结果：ACST时肠道内细菌可通过门静脉向肺脏大量易位，诱发肺功能损伤。ACST的PMP显著增加，并与肺组织中OFR和PLA_2水平呈平行关系，提示OFR和PLA_2在肠源性感染造成的肺损伤中发挥重要作用。中药组的治疗效果最佳，而减压组和氨苄组的治疗效果较差甚至会加重组织的功能损伤。作者认为：不同治疗方法对肺功能的影响与其对肠道细菌易位及肺组织中OFR和PLA_2的影响密切相关。

Effect of different therapies on lung injury in rats with acute cholangitis of severe type

In this study,we observed the bacterial translocationusing (35)~S labelled E. coli as tagged material. Lungpermeability was measured with bovine serum albuminlabelled with (125)~I ;oxygen free radicals (OFR)weredetermined with electron spin resonance spectrometer;and, the activity of phospholipase A_2 (PLA_2 ) was assessedby using biochemical method. The results demonstratedE. coli could translocate to the lung fromthe gut obviously via portal vein which induced a seriouslung injury in the presence of acute cholangitis ofsevere type (ACST ). Lung permeability increased obviouslyin ACST rats. The changes of OFR and PLA_2parelleled the lung injury. Among the three therapies,only Chinese herbs presented an effective protection.Tissue injury was not altered significantly by the internaldrainage of the bile duct or by using ampicillin,and,they seemed even to aggravate tissue injury. Theeffects of different treatments on the pulmonary functionwere closely related to their effects on the bacterialtranslocation and the OFR and PLA_2 in lung tissues.