| 骨桥蛋白在肝包虫动物模型外囊壁中的表达 |
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| 引用本文： | 杨岳,彭心宇,张示杰,李建辉,杨宏强,孙红. 骨桥蛋白在肝包虫动物模型外囊壁中的表达[J]. 农垦医学, 2007, 29(3): 169-171 |
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| 作者姓名： | 杨岳 彭心宇 张示杰 李建辉 杨宏强 孙红 |
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| 作者单位： | 石河子大学医学院;石河子大学医学院第一附属医院普外一科,新疆石河子,832008;浙江大学医学院附属第一医院肝胆胰外科暨器官移植中心,浙江杭州,310003 |
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| 摘 要： | 目的:观察动物模型肝包虫囊壁情况,研究骨桥蛋白(Osteopontin,OPN)在小鼠肝细粒棘球蚴外囊壁中的分布特征及表达相关因素,并探讨其意义.方法:肝包虫动物模型的制作、HE染色观察、免疫组化观察53例小鼠肝细粒棘球蚴外囊壁中OPN及巨噬细胞的表达与分布.结果:HE染色外囊的着色明显较周围巨噬细胞带深,不存在渐变的过程;83%肝细粒棘球蚴外囊壁中有不同程度OPN表达,且集中分布于肝包虫纤维囊壁(外囊),与外侧巨噬细胞带比较有显著差异(P＜0.01).结论:在动物模型上证明了外囊与肝实质间存在可分离间隙,OPN主要分布在肝细粒棘球蚴外囊,并在其形成过程中起重要作用.
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| 关 键 词： | 细粒棘球蚴 动物模型 骨桥蛋白 巨噬细胞 |
Expression of Osteopontin in the Pericystic Layer of Animal Model's Hepatic Hydatid Cyst |
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| Yang yue,Peng xinyu,Zhang shijie,Li jianhui,Yang hongqiang,Sun hong. Expression of Osteopontin in the Pericystic Layer of Animal Model's Hepatic Hydatid Cyst[J]. Agricultural Reclamation Medicine, 2007, 29(3): 169-171 |
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| Authors: | Yang yue Peng xinyu Zhang shijie Li jianhui Yang hongqiang Sun hong |
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| Affiliation: | The First Affiliate of Hospital, Shihezzi university school of medicine, 832005 |
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| Abstract: | Objective: To observe the hydatid cyst in the animal model and to investigate the expression and distribution of osteopontin (OPN) in hydatid cyst and their significances.Methods: 53 mice were infected echinococcus granulosus as to establish the animal model in which the excised cysts were studied with HE and immunohistochemistry staining by using antibodies specific for OPN.Results: It's obviously disparity between hydatid cyst and macrophages in HE.OPN was selectively distributed in the hydatid cyst while it was approximately not found in macrophages.Conclusion Animal model shows that there is a partible interspace between hydatid cyst and liver parenchyma.OPN plays a important role in hydatid cyst's formation mechanism. |
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| Keywords: | animal model osteopontin hydatid cyst |
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