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| IL-4对肺腺癌细胞株ABCG2表达调节的研究 | |
| 引用本文： | 张宇飞,戚好文,赵峰,李召峰,郭丽,穆德广,徐兴祥. IL-4对肺腺癌细胞株ABCG2表达调节的研究[J]. 细胞与分子免疫学杂志, 2007, 23(9): 810-812 |
| 作者姓名： | 张宇飞 戚好文 赵峰 李召峰 郭丽 穆德广 徐兴祥 |
| 作者单位： | 1. 第四军医大学西京医院呼吸内科,陕西,西安,710032;解放军第88医院呼吸内科,山东,泰安,271000 2. 第四军医大学西京医院呼吸内科,陕西,西安,710032 3. 解放军第88医院呼吸内科,山东,泰安,271000 |
| 摘 要： | 目的：探讨IL-4是否影响肺腺癌细胞株ABCG2（ATP-binding cassette supeffamily G member2）的表达。方法：应用半定量RT-PCR检测不同浓度IL-4刺激对肺腺癌细胞株A549和SPC-A-1中ABCG2基因表达的影响。以Western blot检测不同浓度IL-4对A549和SPC-A-1肺腺癌细胞株中AB-CG2蛋白表达的影响。结果：在不同浓度IL-4刺激下肺腺癌细胞株A549和SPC-A-1中ABCG2基因与蛋白的表达无统计学意义（P〉0．05）。结论：ABCG2在腺癌细胞株A549和SPC—A-1中表达，但IL-4对细胞株中ABCG2的表达不具有调节作用。 |
| 关 键 词： | 肺肿瘤 白介素-4 逆转录聚合酶链反应 |
| 文章编号： | 1007-8738（2007）09-0810-03 |
| 修稿时间： | 2007-03-08 |
Effect of IL- 4 on ABCG2 expression in human lung adencarcinoma cell lines |
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| ZHANG Yu-fei,QI Hao-wen,ZHAO Feng,LI Zhao-feng,GUO Li,MU De-guang,XU Xing-xiang. Effect of IL- 4 on ABCG2 expression in human lung adencarcinoma cell lines[J]. Chinese journal of cellular and molecular immunology, 2007, 23(9): 810-812 | |
| Authors: | ZHANG Yu-fei QI Hao-wen ZHAO Feng LI Zhao-feng GUO Li MU De-guang XU Xing-xiang |
| Affiliation: | Department of Respiratory Disease, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. zhang72@fmmu.edu.cn |
| Abstract: | AIM: To investigate the effect of IL-4 on the expression of ABCG2 in lung adencarcinoma cell lines. METHODS: The ABCG2 mRNA and protein expression was determined by semi-quantitative RT-PCR and Western blot respectively. RESULTS: The ABCG2 mRNA and protein was detectable in lung adencarcinoma cell lines A549 and SPC-A-1. But IL-4 stimulation did not significantly affect the ABCG2 mRNA and protein expression in lung adencarcinoma cell lines. CONCLUSION: IL-4 does not regulate ABCG2 expression in human lung adencarcinoma cell lines. |
| Keywords: | ABCG2 |
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