成骨细胞与生物衍生材料联合培养的实验研究

目的：探讨冻干脱钙骨基质（FDBM）作为组织工程骨支架的可行性。方法：取兔颅骨外膜的成骨细胞，经传代培养后作为种子细胞与FDBM于体外联合培养，通过对复合物相差显微镜、光镜及扫描电镜等观察，了解细胞在材料中的生长情况。结果：经系统处理后的FDBM呈现不规则的网－孔结构，其孔隙直径为100－400μm ,孔隙率为70％。体外复合培养8小时，成骨细胞即开始贴附于FDBM网架上；复合培养7天，分布于支架材料上的成骨细胞迅速分化增殖，分泌细胞外基质并形成钙结节。结论：FDBM可作为构建组织工程骨的一种较好支架材料。

EXPERIMENTAL STUDY OF PERIOSTEAL OSTEOBLASTS COCULTURE WITH FREEZE-D RIED DEMINE RALIZED BONE MATRIX

Objective To investigate the feasibility of freeze dried demineralized bone matrix (FDBM) as scaffold material in bone tissue engineering. Methods Osteoblasts which were isolated from cranial periosteum of New Zealand rabbits were cultured as the seeding cells, then the cells were cocultured with heterogenous FDBM in vitro. The cell material complex was observed under phase microscope, light microscope and electronic scanning microscope in order to evaluate the interaction between cells and FDBM. Results Eight hours after coculture, the osteoblasts adhered to FDBM scaffolds. Seven days later, the osteoblasts differentiated and proliferated in FDBM network. Extracellular matrix was secreted and calcium nodes were formed among osteoblasts. Conclusion FDBM is a good scaffold material for the bone tissue engineering.