基质金属蛋白酶-9和血管内皮生长因子在视网膜母细胞瘤中的表达及其临床意义

目的:研究基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)和血管内皮生长因子(vascular endothelial growthfactor,VEGF)在视网膜母细胞瘤(retinoblastoma,RB)中的表达及其与RB分化程度和视神经浸润的关系,探讨它们在RB浸润、转移过程中的作用和临床意义。方法:采用免疫组化方法检测47例RB中MMP-9和VEGF的表达。结果:47例RB中MMP-9和VEGF的阳性表达率分别为46.8%和78.7%。未分化型RB中MMP-9和VEGF的阳性表达均高于分化型(P<0.05);有视神经浸润的RB中MMP-9和VEGF的阳性水平均高于无视神经浸润的RB(P<0.005和P<0.05)。RB中MMP-9和VEGF的表达呈正相关关系(P<0.05)。结论:RB中MMP-9和VEGF高表达与RB的浸润和转移相关,且MMP-9表达与VEGF表达存在相关性。联合检测MMP-9和VEGF的表达可作为反映RB浸润、转移潜能的生物学指标,有助于筛选转移高危病例及评价患者预后。

Expression of matrix metalloproteinase-9 and vascular endothelial growth factor in retinoblastoma and its clinical significance

Objective: To study the expression of matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor(VEGF) in retinoblastoma(RB) and its relationship with the differentiation and optic nerve infiltration of RB,and to investigate the role of MMP-9 and VEGF in the infiltration and metastasis of RB and their clinical significance.Methods: Immunohistochemical technique was used to detect the protein expression of MMP-9 and VEGF in 47 cases.Results: The positive rate of MMP-9 protein and VEGF protein expression in 47 cases were 46.8% and 78.7%,respectively.The expression of MMP-9 and VEGF was significantly higher in the undifferentiated pattern than in the rosetted pattern(P<0.05);and the expression of MMP-9 and VEGF was significantly higher in tumors with optic nerve invasion than in those without optic nerve invasion(P<0.005 and P<0.05).In RB,the expression of MMP-9 had a positive correlation with the expression of VEGF.Conclusions: In RB,high expression of MMP-9 and VEGF is related to the invasion and metastasis of RB.The expression of MMP-9 has a positive correlation with the expression of VEGF.The expression of MMP-9 and VEGF may act as a biological marker for the invasion and metastasis of RB,and might be helpful to filter cases of high risk for metastasis and to predict prognosis of the patients.