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可注射性纳米组织工程骨的生物相容性
引用本文:王明海,冯庆玲,董有海,陈垒,洪洋,吴俊国,钱光. 可注射性纳米组织工程骨的生物相容性[J]. 中华实验外科杂志, 2009, 26(3). DOI: 10.3760/ema.j.issn.1001-0030.2009.03.026
作者姓名:王明海  冯庆玲  董有海  陈垒  洪洋  吴俊国  钱光
作者单位:1. 复旦大学附属上海市第五人民医院骨科,上海,200240
2. 清华大学材料科学与工程系
基金项目:上海市科委科技攻关计划资助项目 
摘    要:目的 以可注射性纳米材料与共培养的成骨细胞和血管内皮细胞复合,构建可注射性组织工程骨,并观察其体外实验的生物相容性.方法 将共培养的成骨细胞和血管内皮细胞与可注射性纳米材料体外复合培养,噻唑蓝(MTT)比色法测定细胞活性,检测碱性磷酸酶(ALP)活性,并行激光共聚焦显微镜及扫描电镜观察,进行形态学和功能测定.结果 细胞复合材料后保持正常的分裂增殖速度,细胞的ALP活性与单纯细胞培养的对照组差异无统计学意义(P>0.05).激光共聚焦显微镜及扫描电镜观察可见,细胞能在可注射性纳米材料上良好地增殖、生长,细胞的活性未受到材料的影响.结论 可注射性纳米材料具有良好的细胞相容性,可作为骨组织工程可注射性载体材料.

关 键 词:组织工程  成骨细胞  内皮细胞  羟基磷灰石  生物相容性

Biocompatibility of the injectable nano tissue-engineered bone
Abstract:Objective To explore the biocompatibility of the injectable nano tissue-engineered bone by co-culturing with the osteoblasts and vascular endothelial cells in vitro.Methods The morpholog-ic features and function of the co-cultured ceils with the injectable NHAC (nano-HAP/Collagen) in vitro were observed by laser scanning confocal microscopy (LSCM), scanning electron microscopy (SEM) and methods of MTF and ALP assay respectively.Results The ceils co-cultured with the injectable NHAC could preserve normal proliferative speed and ALP value showed no difference as compared with control groups.LSCM and SEM revealed the co-cultured cells could proliferate and grew normally in the injectable NHAC and the cellular activity was not influenced by the injectable NHAC.Conclusion The injectable NHAC has good biocompatibility and can be used as biomaterial in bone tissue engineering.
Keywords:Tissue engineering  Osteoblast  Endothelial cells  Hydroxyapatite  Biocompatibility
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