miR－320a表达与结直肠癌患者预后的关系

目的：探讨miR－320a与结直肠癌患者的临床病理特征及预后的相关性。方法选取2010年1月至2013年3月在本科室收治的65例已经配对的癌旁正常组织和结直肠癌组织，采用实时定量PCR的方法检测癌旁正常组织和癌组织中miR－320a的表达情况，分析miR－320a表达情况与结直肠癌患者的临床病理特点及生存预后的关系。结果结直肠癌组织中miR－320a表达量明显低于癌旁正常组织（P＜0．001）。结直肠癌患者组织中miR－320a的表达与肿瘤分化程度、TNM分期和CEA水平有关（均P＜0．05），而与年龄、性别、肿瘤部位无关（均P＞0．05）。生存分析结果显示miR－320a高表达的结直肠癌患者术后总体预后明显优于miR－320a低表达者，两组患者的5年生存率分别为54．7％和31．4％（P＝0．022）。结论 miR－320a 可能作为结直肠癌患者预后的预测指标。miR－320a过表达可能提示结直肠癌患者肿瘤生物学行为较佳。

The relationship between miR-320a expression and prognosis in patients with colorectal cancer

Objective To investigate the relationship between miR-320a expression and clinicopathological features and prognosis of colorectal cancer patients. Methods The expression levels of miR-320a in matched colorectal cancer and adjacent normal tissue from 65 colorectal cancer patients from January 2010 to March 2013 in our department was evaluated with real-time PCR. Clinicopathological features and prognosis of these patients was also analyzed to find out the clinical effect of miR-320a in colorectal cancer. Results We found that the relative expression of miR-320a was lower in colorectal cancer compared with adjacent normal tissue (P<0.001). In addition, the expression of miR-320a was associated with histological grade, TNM stage and CEA level (all P<0.05), while no correlation was found in terms of age, sex and tumor location(all P>0.05). Moreover, patients with miR-320a overexpression had a better postoperative survival outcome compared with those with lower expression (5-year survival rate:54.7% vs. 31.4%,P=0.022). Conclusions miR-320a presents as a potential biomarker for the prognosis of colorectal cancer patients , and overexpression of miR-320a may be associated with better tumor biological behavior in colorectal cancer.