神经干细胞对胶质瘤细胞抑制作用的实验研究

目的:探讨神经干细胞在体外对胶质瘤细胞有无抑制作用。方法:取胎鼠大脑皮层组织,于无血清培养基中进行细胞培养成神经干细胞并进行Nestin免疫荧光染色鉴定;将神经干细胞和鼠胶质瘤细胞(C6细胞)在体外混合培养,观察肿瘤细胞的生长、增殖情况及形态学变化,并对C6细胞进行FCM细胞周期分析及MTT试验。结果:培养所得的细胞特异性抗原Nestin染色呈现阳性,具有较强的分裂增殖能力和自我更新能力,表明培养所获得的细胞是神经干细胞;神经干细胞与C6细胞混合培养后,C6细胞不易贴壁,增殖慢。FCM分析显示实验组处于G0/G1期的C6细胞明显多于对照组(χ2=14.77,P<0.01)。MTT试验显示实验组光吸收值低于对照组(t=-11.66,P<0.01)。结论:胚鼠大脑皮层组织可培养出神经干细胞;在体外对胶质瘤细胞的生长有抑制作用。

The research on restriction of neural stem cells to gliomas cells

Objective: To research the restriction of neural stem cells to gliomas cells (C6) in vitro. Methods: Cells from the embryonic Sprague-Dawley rat (SD rat) cerebral cortices were cultured in the serum -free medium and identified by immunocyto chemical procedures. Neural stem cells from the first step were mixed with gliomas cells (C6), then cultured together, the changes of gliomas cells were observed by the inverted microscope and then analyzed by the flow cytometre and tested by MTT. Results: Neural stem cells were successfully cultured from embryonic SD rat cerebral cortices stained by anti-nestin.Neural stem cells had the ability of proliferation and clonality,when cocultuned with gliomas cells.Gliomas cells grew multiplied and attached to the bottom slowly .Flow cytometre analysis showed that percentage of gliomas cells in G0/G1 from the experiment group were more than in the control group(P< 0.01). MTT test showed absorbances of the gliomas cells were different in the experiment group and the control group (P <0.01). Conclusion: Neural stem cells can be cultured and isolated from embryonic SD rat cerebral cortices. Neural stem cells exert restriction to gliomas cells(C6) in vitro.