MBP-SF, a prominent serum factor in suckling Lewis rats that additively inhibits the primary binding of myelin basic protein (MBP) to syngeneic anti-MBP antibodies. |
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| Authors: | E D Day V A Varitek R S Fujinami P Y Paterson |
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| Affiliation: | 1. Departments of Microbiology-Immunology and Surgery. Duke University Medical Center. Durham, NC 27710, U.S.A.;2. Department of Microbiology-Immunology. The Medical and Dental Schools, Northwestern University, Chicago, IL 60611. U.S.A.;1. School of Geography and Planning, Sun Yat-Sen University, Guangzhou 510275, China;2. Guangzhou Marine Geological Survey, Guangzhou 510760, China;1. Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, Shandong 250022, China;2. School of Material Science and Engineering, University of Jinan, Jinan, Shandong 250022, China;3. Building Materials and Construction Chemistry, Technology University of Berlin, Gustav-Meyer-Allee 25, Berlin 13355, Germany;1. Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico;2. Tecnológico de Monterrey, Escuela de Medicina y Ciencias de la Salud, Ave. Morones Prieto 3000, Monterrey, N.L., México 64710, Mexico;1. Organ Transplant Centre of Excellence, King Faisal Specialist Hospital and Research Centre Riyadh, Saudi Arabia;2. Comparative Medicine Department, King Faisal Specialist Hospital and Research Centre Riyadh, Saudi Arabia;3. College of Medicine, Alfaisal University, Riyadh, Saudi Arabia;1. Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario “A. Gemelli”, IRCCS, Largo A. Gemelli 8, 00168 Roma, Italy;2. Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie – Sezione di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Roma, Italy;3. Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie – Sezione di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Roma, Italy;4. Dipartimento di Scienze della Salute della Donna, del Bambino e di Sanità Pubblica, Fondazione Policlinico Universitario “A. Gemelli”, IRCCS, Largo A. Gemelli 8, 00168 Roma, Italy;5. Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy;6. Department of Health Science, University “Magna Græcia” of Catanzaro, Viale Europa, 88100 Catanzaro, Italy;7. Fondazione Policlinico Universitario “A. Gemelli”, IRCCS, Largo A. Gemelli 8, 00168 Roma, Italy;8. Dipartimento di Scienze della Vita e Sanità Pubblica - Sezione di Ginecologia ed Ostetricia, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Roma, Italy |
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| Abstract: | MBP-SF2 is a serum factor in suckling Lewis rats that additively inhibits the binding of125I-labeled and unlabeled myelin basic protein (MBP) to syngeneic Lewis rat anti-MBP antibodies. It can be detected and measured by 4 different methods of immunological inhibition analysis involving 2 different approaches to radioimmunoassay (salting-out with sodium sulfate, precipitation with rabbit anti-rat immunoglobulin light chain). Thus. MBP-SF contains all the determinants of Lewis rat MBP that are reactive with syngeneic anti-MBP antibodies. Analyses of 47 pools of suckling and control serums, drawn from many different litters at different times, indicate a general rise in activity to a mean peak value of 14ng/μl within 2 weeks after birth. The serum factor is still present in weanling rats but decreases to insignificant levels by the 6th postnatal week. In the pooled serum of one litter 12 ng MBP-SF/μl were present at birth before suckling began. There are at least three hypotheses which can account for the presence of MBP-SF: the factor may (A) represent MBP that is synthesized in excess over that required for the expanding myelin “sink” during the postnatal period; (B) comprise only those peptide regions of MBP whose determinants are involved in humoral immunity: or (C) consist of proteins or peptides of non-neural origin that share the MBP determinants involved in humoral immunity. Regardless of which hypothesis is correct, the cross-reaction of MBP-SF with anti-MBP. coupled with its prominence in early postnatal development, would seem to suggest that it may act as a circulating immunoregulatory molecule or endogenous autoneurotolerogen for humoral immunity to MBP determinants. |
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