| 恶性疟原虫乳酸脱氢酶活性测定及在药物疗效监测方面的初步观察 |
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| 引用本文： | 吴英松,李明,陆家海,毕惠祥,李英杰.恶性疟原虫乳酸脱氢酶活性测定及在药物疗效监测方面的初步观察[J].南方医科大学学报,2002,22(1):25-27. |
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| 作者姓名： | 吴英松 李明 陆家海 毕惠祥 李英杰 |
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| 作者单位： | 1. 第一军医大学热带病研究室,广东,广州,510515
2. 中山医科大学寄生虫学教研室,广东,广州,510080 |
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| 基金项目： | 全军医药卫生科研基金(96L034) |
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| 摘 要： | 目的探讨疟原虫乳酸脱氢酶(Plasmodiumlactatedehydrogenase,pLDH)活性与原虫密度、培养时间的关系,以及在监测药物疗效等方面的作用。方法利用酶比色法对不同原虫密度、不同培养时间以及药物治疗前后患者的血样进行pLDH活性检测。结果pLDH的活性随恶性疟原虫感染率的提高、培养时间的增加而明显地升高,最大活性是在虫体培养36~48h之间,虫体主要处在裂殖体期和滋养体期;混合感染(恶性疟原虫和间日疟原虫)患者经药物治疗后血液中pLDH活性明显降低,治疗5d后已检测不到pLDH的活性。结论pLDH活性检测对于疟原虫克隆株鉴定、药物的疗效监测等方面具有一定的应用价值。
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| 关 键 词： | 恶性疟原虫 乳酸脱氢酶 活性测定 |
| 文章编号： | 1000-2588(2002)01-0025-03 |
| 修稿时间： | 2001年4月30日 |
Plasmodium lactate dehydrogenase activity assay and its preliminary application in therapeutic efficacy monitoring of drugs |
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| WUYing-song ,LI Ming ,LU Jia-hai ,BIHui-xiang ,LI Ying-jie Instituteof TropicalDiseases,FirstMilitaryMedicalUniversity,Guangzhou,China,.Plasmodium lactate dehydrogenase activity assay and its preliminary application in therapeutic efficacy monitoring of drugs[J].Journal of Southern Medical University,2002,22(1):25-27. |
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| Authors: | WUYing-song LI Ming LU Jia-hai BIHui-xiang LI Ying-jie Instituteof TropicalDiseases FirstMilitaryMedicalUniversity Guangzhou China |
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| Institution: | WUYing-song 1,LI Ming 1,LU Jia-hai 2,BIHui-xiang 1,LI Ying-jie 11 Instituteof TropicalDiseases,FirstMilitaryMedicalUniversity,Guangzhou510515,China,2 Departmentof Parasi-tology,SunYat-senUniversityof MedicalSciences,Gu |
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| Abstract: | Objective To evaluatetheactivityof Plasmodium lactatedehydrogenase(pLDH)inrelationto thedegreeof parasit-emiaandlaboratoryculturetimeof theparasite,andexplorethefeasibilityof usingtheactivityassessmentinmonitoringthe effectof drugtherapies.Method Usingenzymaticcolorimetricmethod,pLDHactivitieswereassayed in thebloodsamples of differentdegreesof parasitemiaandof variedin vitro culturetime.Bloodsamplesfroma patientwithP.falciparum andP.vivax infectionwerealsocollectedbeforeandafterchemotherapyforpLDHactivityassay.Results pLDHactivityincreased as parasitemiaescalatedandtheculturetimeprolonged.Thestrongestactivitywasobservedbetween36and48h of incubation whenthetrophozoitesandschizontswerepredominant.pLDHactivitieswerereducedin thebloodsampleof thepatient collectedafter chemotherapy,completelyundetectableafter5d.Conclusion pLDHactivityassaywouldpotentiallybe of valueindetectingspecificPlasmodium clonesandmonitoringefficacyof drugtherapy. |
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| Keywords: | Plasmodium falciparum lactatedehydrogenase activityassay |
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