| Silencing effect of recombinant plasmids PPARγ-pSUPER-EGFP on the expression of peroxisome proliferator-activated receptorγ |
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| 引用本文： | 杨策,王正国,陈力勇,周健,朱佩芳,蒋建新.Silencing effect of recombinant plasmids PPARγ-pSUPER-EGFP on the expression of peroxisome proliferator-activated receptorγ[J].中华创伤杂志(英文版),2005,8(6):352-357. |
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| 作者姓名： | 杨策 王正国 陈力勇 周健 朱佩芳 蒋建新 |
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| 作者单位： | State Key Laboratory of Trauma Burns and Combined Injury Department 4，Institute of Surgery Research Daping Hospital，Third Military Medical University，Chongqing 400042，China，State Key Laboratory of Trauma，Burns and Combined Injury Department 4，Institute of Surgery Research Daping Hospital，Third Military Medical University，Chongqing 400042，China，State Key Laboratory of Trauma，Burns and Combined Injury Department 4，Institute of Surgery Research Daping Hospital，Third Military Medical University，Chongqing 400042，China，State Key Laboratory of Trauma，Burns and Combined Injury Department 4，Institute of Surgery Research Daping Hospital，Third Military Medical University，Chongqing 400042，China，State Key Laboratory of Trauma，Burns and Combined Injury Department 4，Institute of Surgery Research Daping Hospital，Third Military Medical University，Chongqing 400042，China，State Key Laboratory of Trauma，Burns and Combined Injury Department 4，Institute of Surgery Research Daping Hospital，Third Military Medical University，Chongqing 400042，China |
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| 基金项目： | This work was supported Laboratory of Trauma, Burns Military Medical University by 0pen Fund of State Key and Combined Injury, Third We thank Dr. Wan Qi for discussions about RNAi and pSUPER expression vectors. We are grateful to Dr. Zhou Yuanguo for providing plasmids. |
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| 摘 要： | Peroxisome proliferator-activated receptor γ( PPARγ), belonging to the nuclear steroidreceptor superfamily, plays an important role in1,2lipid metabolism and glucose homeostasis.1,2Recently,some studies have shown that PPARγcan specificallysuppress the gene expression of pro-inflammatorymediators through transactivation, as a checkpoint incontrolling the inflammatory responses. PPARγ iswidely distributed on macrophages, dendritic cells, Tcells, B cells and so on. Its expression is dra…
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| 关 键 词： | 噪声抑制 重组细胞 质粒 PPARγ-pSUPER-EGFP 过氧物酶体 姜黄色素 脂多糖 |
| 收稿时间： | 2004-11-02 |
Silencing effect of recombinant plasmids PPARγ-pSUPER-EGFP on the expression of peroxisome proliferator-activated receptorγ |
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| YANG Ce,WANG Zheng-guo,CHEN Li-yong,ZHOU Jian,ZHU Pei-fang,JIANG Jian-xin.Silencing effect of recombinant plasmids PPARγ-pSUPER-EGFP on the expression of peroxisome proliferator-activated receptorγ[J].Chinese Journal of Traumatology(English Edition),2005,8(6):352-357. |
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| Authors: | YANG Ce WANG Zheng-guo CHEN Li-yong ZHOU Jian ZHU Pei-fang JIANG Jian-xin |
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| Institution: | State Key Laboratory of Trauma, Bums and Combined Injury, Department 4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China |
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| Abstract: | OBJECTIVE:To investigate the silencing effect of gene encoding peroxisome proliferator-activated receptor gamma (PPARgamma) on the expression of tumor necrosis factor alpha (TNFalpha) by constructing vectors for RNA interference in RAW264.7 cells. METHODS:The pSUPER-EGFP vectors were used to transcribe functional small interfering RNA (siRNA). Four pairs of oligonucleotides (64 nt) targeting PPARgamma gene were inserted into the downstream of the H1 promotor, with their veracity confirmed by double digestion and sequencing. Western blotting and immunofluorescence assay were used to examine the silencing effect of PPARgamma gene in RAW264.7 cells. Meanwhile, the TNFalphalevel was determined by Sandwich ELISA. RESULTS:Compared with other recombinant pSUPER-EGFP vectors (R-pSUPER.EGFP), R-pSUPER.EGFP2 induced the best silencing effect on the expression of PPARgamma in RAW264.7 cells, which played an obvious inhibitory role in down-regulating the TNFalphaexpression after the curcumin and lipopolysaccharide (LPS) stimulation. CONCLUSIONS: PPARgamma-pSUPER-EGFP inducing a silencing effect on the expression of PPARgamma can efficiently play a negative role in controlling the inflammatory responses of RAW264.7 cells. |
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| Keywords: | PPAR alpha RNA small interfering Tumor necrosis factor- alpha |
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