Synthesis and purification of the aflatoxin B1-lysine adduct |
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| Authors: | Daiane Cristina Sass Alessandra Vincenzi Jager Fernando Gustavo Tonin Roice Eliana Rosim Mauricio Gomes Constantino |
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| Institution: | 1. Department of Food Engineering, School of Animal Science and Food Engineering, University of S?o Paulo, Pirassununga, SP, Brazil,;2. Department of Biossystem Engineering, School of Animal Science and Food Engineering, University of S?o Paulo, Pirassununga, SP, Brazil, and;3. Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, SP, Brazil |
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| Abstract: | This work reports the chemical synthesis of aflatoxin B1 (AFB1)-lysine based on procedures available in the literature, but using lysine without a protection group in the α-amine group. AFB1-exo-8,9-epoxide was obtained by epoxidation of AFB1 with chloroperoxybenzoic acid in dichloromethane and phosphate buffer. Purification and identification of the AFB1-lysine were conducted by liquid chromatography (LC), and its structure was confirmed by LC with mass spectrometer and diode-array detection. The preparation of AFB1-lysine using lysine without a protection group in the α-amine group was completed in 24?h, being a practical modification of available methods that can be reproduced in analytical laboratories. |
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| Keywords: | AFB1-lysine aflatoxins biomarkers synthesis |
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