A comparison of the mouse bioassay with liquid chromatography-mass spectrometry for the detection of lipophilic toxins in shellfish from Scottish waters. |
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Authors: | E A Turrell L Stobo |
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Affiliation: | Fisheries Research Services, Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK. turrelle@marlab.ac.uk |
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Abstract: | Some lipophilic shellfish toxins (LSTs) can cause human illness due to eating shellfish that have become naturally contaminated following filter feeding on toxin producing algae. A mouse bioassay (MBA) is widely used to detect LSTs in regulatory monitoring of shellfish. However, the MBA is imprecise giving only a positive or negative result and is prone to interference from other compounds. In this study, liquid chromatography-mass spectrometry (LC-MS) was compared to the MBA, with the aim of substituting the in vivo assay for monitoring shellfish from Scottish waters. Overall, it was not feasible to demonstrate equivalence of LC-MS with the MBA, but due to the detection of a range of LSTs, it is considered that LC-MS methods capable of detecting multiple analogues are accepted by international markets of shellfish to assure consumer protection. |
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Keywords: | Mouse bioassay Diarrhetic shellfish poisoning (DSP) Lipophilic shellfish toxins (LSTs) Liquid chromatography-mass spectrometry (LC-MS) |
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