抑癌基因PTEN在宫颈浸润癌和癌前病变组织中的表达及意义

目的:探讨抑癌基因PTEN在宫颈癌及癌前病变组织中的表达及意义。方法:选取110例石蜡包埋宫颈病变组织,其中慢性宫颈炎组织30例,宫颈上皮内瘤变(cervical intraepithelial neoplasia CIN)Ⅰ、Ⅱ、Ⅲ级组织分别为25、15、10例,浸润性宫颈鳞癌组织30例(Ⅰ、Ⅱ、Ⅲ～Ⅳ期各10例)。应用免疫组化方法检测PTEN蛋白在上述组织中的表达。结果:PTEN在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ、浸润性宫颈鳞癌组织中,阳性表达率分别为100.00%、92.00%、73.33%、40.00%、36.67%,表达率逐渐下降,差别有统计学意义(P0.05)。PTEN表达与宫颈鳞癌病理分期有关(P0.05)。结论:PTEN与宫颈癌的发生、发展有关。

Expression and significance of tumor suppressor gene PTEN in cervical invasive carcinoma and cervical precancerous lesion

Objective:To explore the expression and significance of tumor suppressor gene PTEN in cervical invasive carcinoma and cervical precancerous lesion.Methods:110 paraffin-embedded cervical lesions were selected,including 30 cases of chronic cervicitis,25 cases of cervical intraepithelial neoplasia (CIN) Ⅰ,15 cases of CIN Ⅱ,10 cases of CIN Ⅲ,30 cases of invasive cervical squamous cell cancer (10 cases of stage Ⅰ,10 cases of stage Ⅱ,10 cases of stage Ⅲ～Ⅳ).The expression levels of PTEN in the 110 cases were detected by immunohistochemical method.Results:The positive expression rates of PTEN in chronic cervicitis,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and invasive cervical squamous cell cancer were 100.00%,92.00%,73.33%,40.00% and 36.67%,respectively.The positive expression rates of PTEN in these specimens descended gradually,there was significant difference (P<0.05).There was a correlation between PTEN expression and pathological stages of squamous cell cancer (P<0.05).Conclusion:PTEN participates in the occurrence and development of cervical carcinoma.