维生素A和锌过量摄入对幼龄大鼠脾脏抗氧化能力的影响

目的:探讨维生素A(VitA)和锌(Zn)摄入过量对幼龄大鼠脾脏抗氧化能力的影响,并为VitA和Zn的营养补充上限的制定提供实验室参考依据。方法:通过灌胃造成VitA摄入10倍于推荐摄入量(RNI)的大鼠动物模型,摄入剂量为0.48g/kg,Zn摄入20倍于RNI的大鼠动物模型,摄入剂量为1.00g/kg,并按相同剂量设立联合过量组,同时设立对照组。在实验第4周末处死全部大鼠,制备脾脏组织匀浆,测定大鼠脾脏总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量。应用SPSS15.0统计软件对其测定结果进行统计分析。结果:实验组脾脏中T-AOC、SOD、GSH-Px、CAT活性明显降低,MDA含量显著升高,与对照组相比差异有统计学意义(P0.05),而且VitA和Zn联合过量组较之VitA和Zn单一过量组差异更明显。结论:过量补充VitA和Zn可降低幼龄大鼠脾脏的抗氧化能力。

Effect of excessive intake of vitamin A and Zinc(Zn)on the antioxidant capacity of young rats' spleen

Objective:To investigate the effect of excessive intake of vitamin A and Zinc (Zn) on the antioxidant capacity of young rats' spleen,and to provide the laboratory reference of the upper limit of the vitamin A and Zinc's nutritional supplement.Methods:Instill 0.48 g/kg dose VA into the rats'stomach resulted one animal model which 10 times VA's RNI.And 1.00 g/kg dose Zn resulted another animal model which 20 times Zn's RNI.According to the same dose,a joint excessive group of VA and Zn was established.At the same time a contol group was established.Kill all the rats at the end of 4th weekend of the experiment.And the spleen tissue homogenate was prepared,the rats' spleen total antioxidant capacity (T-AOC),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),catalase (CAT) and malondialdehyde vitality (MDA) were determined.The statistic results were analyzed with SPSS15.0 software.Results:The spleen' s T-AOC,SOD,GSH-Px,CAT activity decreased significantly,and MDA was significantly increased in experiment group.Compared with the contol group,there were significantly difference (P < 0.05).And the differents were more significant between the joint excessive group of VA and Zn and the separate excessive group.Conclusion:The excessive supplementary of VA and Zn can reduce the antioxidant capacity of the rats' spleen.