| TRAIL对人乳腺癌、宫颈癌、卵巢癌细胞抗肿瘤作用的实验研究 |
| |
| 引用本文： | 闻静,张阳. TRAIL对人乳腺癌、宫颈癌、卵巢癌细胞抗肿瘤作用的实验研究[J]. 中国妇幼保健, 2011, 26(5): 747-748 |
| |
| 作者姓名： | 闻静 张阳 |
| |
| 作者单位： | 1. 吉林省长春市妇产医院,130042
2. 吉林省肿瘤防治研究所 |
| |
| 基金项目： | 吉林省科技厅资助项目〔20070545〕 |
| |
| 摘 要： | 目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人乳腺癌(MCF-7)、宫颈癌(Hela)、卵巢癌(SKOV-3)细胞体外抗肿瘤的杀伤作用。方法:通过MTT法、流式细胞术检测重组人TRAIL蛋白对3种肿瘤细胞和人胚肾(HEK-293)正常细胞系的作用和诱导凋亡情况。结果:一定浓度的TRAIL可有效抑制MCF-7、SKOV-3、Hela细胞,其生长抑制呈剂量依赖。TRAIL对SKOV-3细胞最敏感,用2.5 mg/L TRAIL作用SKOV-3,12h后发生凋亡,其细胞凋亡率呈时间依赖。结论:重组人TRAIL对人乳腺癌、宫颈癌、卵巢癌细胞系具有生长抑制作用,并能够诱导SKOV-3细胞凋亡。
|
| 关 键 词： | TRAIL 凋亡 恶性肿瘤 |
Experimental study on anti-tumor effect of TRAIL on human breast cancer, cervical carcinoma and ovarian carcinoma |
| |
| WEN Jing,ZHANG Yang. Experimental study on anti-tumor effect of TRAIL on human breast cancer, cervical carcinoma and ovarian carcinoma[J]. Maternal and Child Health Care of China, 2011, 26(5): 747-748 |
| |
| Authors: | WEN Jing ZHANG Yang |
| |
| Affiliation: | WEN Jing,ZHANG Yang.Hospital for Gynecology and Obstetrics,Changchun 130042,Jilin,China |
| |
| Abstract: | Objective:To explore the anti-tumor effect of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) on human breast cancer(MCF-7 cells),cervical carcinoma(HeLa cells) and ovarian carcinoma(SKOV-3 cells).Methods:MTT assay and flow cytometry were used to detect the effect of recombination human TRAIL protein on 3 kinds of tumor cells and human embryo kidney normal cell line(HEK-293 cells) and apoptosis-inducing condition.Results:TRAIL of certain concentration could inhibit MCF-7,SKOV-3 and HeLa cells... |
| |
| Keywords: | TRAIL |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
