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| 苯丁酸钠对宫颈癌Hela细胞增殖及p21WAF1/CIP1和CDK7表达的影响 | |
| 引用本文： | 罗晓华,许雅娟,崔金全,张占薪,李梅.苯丁酸钠对宫颈癌Hela细胞增殖及p21WAF1/CIP1和CDK7表达的影响[J].中国妇幼保健,2011,26(4):604-607. |
| 作者姓名： | 罗晓华 许雅娟 崔金全 张占薪 李梅 |
| 作者单位： | 1. 郑州大学第三附属医院妇产科,河南郑州,450052 2. 郑州大学第二附属医院妇产科 3. 郑州人民医院妇产科 |
| 摘 要： | 目的:探讨苯丁酸钠(SPB)在体外诱导宫颈癌Hela细胞生长抑制和细胞周期阻滞以及对p21WAF1/CIP1和CDK7基因表达的影响。方法:体外培养Hela细胞,应用MTT法检测苯丁酸钠对Hela细胞增殖的影响,流式细胞仪分析细胞周期的变化,半定量RT-PCR法检测细胞p21WAF1/CIP1和CDK7基因表达水平。结果:苯丁酸钠明显抑制Hela细胞增殖,呈时间-剂量依赖性;苯丁酸钠诱导Hela细胞周期阻滞于G0/G1期,使S期细胞数减少;苯丁酸钠促进抑癌基因p21WAF1/CIP1的表达,对CDK7基因的表达有抑制作用。结论:苯丁酸钠在体外抑制宫颈癌Hela细胞的增殖,使之阻滞于G0/G1期,可能与上调p21WAF1/CIP1基因表达、下调CDK7基因表达有关。 |
| 关 键 词： | Hela细胞 组蛋白去乙酰化酶抑制剂 苯丁酸钠 细胞增殖 细胞周期 |
Effect of sodium phenylbutyrate on proliferation of cervical cancer HeLa cells and expressions of D21 WAF1/CIPI and CDK7 |
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| LUO Xiao-Hua,XU Ya-Juan,CUI Jin-Quan et al.Effect of sodium phenylbutyrate on proliferation of cervical cancer HeLa cells and expressions of D21 WAF1/CIPI and CDK7[J].Maternal and Child Health Care of China,2011,26(4):604-607. | |
| Authors: | LUO Xiao-Hua XU Ya-Juan CUI Jin-Quan |
| Institution: | LUO Xiao-Hua,XU Ya-Juan,CUI Jin-Quan et al.Department of Obstetrics and Gynecology,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China |
| Abstract: | Objective:To explore the effect of sodium phenylbutyrate on proliferation and cell cycle arrest of cervical cancer HeLa cells and expressions of p21WAF1/CIP1 gene and CDK7 gene.Methods:HeLa cells were cultured in vitro,MTT method was used to detect the effect of sodium phenylbutyrate on proliferation of cervical cancer HeLa cells,flow cytometry was used to analyze the changes of cell cycles,and semi-quantitative RT-PCR was used to detect the expression levels of p21WAF1/CIP1 gene and CDK7 gene.Results:Sodiu... |
| Keywords: | HeLa cells Histone deacetylase inhibitor Sodium phenylbutyrate Cell proliferation Cell cycle |
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