TRAIL基因多态性与妊娠期糖尿病的相关性

目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因1 525位点G/A、1 595位点C/T单核苷酸多态性(SNP)与妊娠期糖尿病(GDM)的关系及TRAIL在GDM发生中的可能作用。方法:利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)的方法检测TRAIL基因1 525位点G/A、1 595位点C/T多态性,并测定空腹血糖(FPG)、空腹胰岛素(FINS)等生化指标。计算相应的基因型频率和等位基因频率,通过χ2检验和方差分析,分析TRAIL基因型和等位基因与GDM及各生化指标的相关性。结果:GDM组TRAIL基因型频率和等位基因频率高于正常妊娠组(分别为P<0.05和P<0.01);GDM组GA/CT、AA/TT基因型FPG、FINS均高于GG/CC基因型(P<0.05)。结论:TRAIL基因1 525位点G/A、1 595位点C/T单核苷酸多态性与妊娠期糖尿病密切相关,可能参与GDM的发病机制。

Association of TRAIL gene polymorphism with gestational diabetes mellitus

Objective:To investigate the effect of single nucleotide polymorphism(SNP) of TRAIL(Tumor necrosis factor-related apoptosis inducing ligand) gene at sites 1525A/G and 1595C/T on GDM susceptibility,to explore the potential roles of TRAIL involved in the development of GDM. Methods:The genotype and allele frequencies of TRAIL gene at sites 1525A/G and 1595C/T in patients with GDM and healthy controls were detected with polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).FPG,FINS etc were measured in all subjects in GDM group.Chi-square test and one-way ANOVA were used to analyze the relationship of TRAIL genotype and alleles with GDM and related bioparameters. Results:Frequencies of TRAIL genotypes and allele frequencies at site 1525 and 1595 in GDM patients were obviously higher than that in healthy controls.The levels of FPG and FINS in GDM patients with AA/CC or AG/CT genotype were significantly higher than those with GG genotype. Conclusion:SNPs of TRAIL at 1525 A/G and 1595 C/T have association with gestational diabetes mellitus.TRAIL probably involves in the cause of GDM.