| 塞来昔布对宫颈癌HeLa细胞的放射增敏作用及影响因素的研究 |
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| 引用本文： | 田晓予,王爱红,余娟娟,刘华. 塞来昔布对宫颈癌HeLa细胞的放射增敏作用及影响因素的研究[J]. 中国妇幼保健, 2011, 26(6): 912-915 |
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| 作者姓名： | 田晓予 王爱红 余娟娟 刘华 |
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| 作者单位： | 河南科技大学第一附属医院妇产科,河南,洛阳,471003 |
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| 基金项目： | 河南省洛阳市科技攻关项目 |
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| 摘 要： | 目的:研究塞来昔布(Celecoxib)对人宫颈癌HeLa细胞放射增敏作用以及给药顺序对放射增敏作用的影响。方法:采用MTT法测定塞来昔布作用于HeLa细胞的半数抑制浓度(IC50),应用克隆形成和单击多靶数字模型检测塞来昔布的放射增敏作用及不同给药顺序的细胞存活率及放射增敏比(SER)。结果:塞来昔布对宫颈癌Hela细胞株增殖有抑制作用,其作用呈现剂量-效应和时间-效应依赖性,塞来昔布对Hela细胞的IC50为44μmol/L,取40μmol/L塞来昔布联合X射线照射作用于Hela细胞,先给药后照射组SER为2.46,给药中照射组SER为1.91,先照射后给药组SER为1.44;免疫化学实验证实塞来昔布可下调Hela细胞VEGF的表达。结论:塞来昔布对Hela细胞具有放射增敏作用,其增敏作用有顺序依从性;其机制可能是通过下调人宫颈癌Hela细胞的VEGF表达而实现。
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| 关 键 词： | 塞来昔布 Hela细胞 放射增敏 VEGF |
Study on radiosensitization effect of celecoxib on human cervical cancer HeLa cells and the effect factors |
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| TIAN Xiao-Yu,WANG Ai-Hong,YU Juan-Juan et al. Study on radiosensitization effect of celecoxib on human cervical cancer HeLa cells and the effect factors[J]. Maternal and Child Health Care of China, 2011, 26(6): 912-915 |
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| Authors: | TIAN Xiao-Yu WANG Ai-Hong YU Juan-Juan et al |
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| Affiliation: | TIAN Xiao-Yu,WANG Ai-Hong,YU Juan-Juan et al.Department of Gynecology and Obstetrics,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,Henan,China |
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| Abstract: | Objective:To study the radiosensitization effect of celecoxib on human cervical cancer HeLa cells and the effect of administration sequence on radiosensitization.Methods:MTT method was used to detect the inhibitory concentration 50(IC 50) of Hela cells after treated with celecoxib,clone formation and single-hit multitarget model detection were used to detect the radiosensitization effect of celecoxib,cell survival rate and sensitive enhancement ratio(SER) of different administration sequences.Results:Celeco... |
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| Keywords: | VEGF |
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