女性生殖道乳杆菌的分离及鉴定

目的:从正常女性生殖道中分离乳杆菌,为开发复方益生菌制剂提供新型菌株。方法:采用传统方法鉴定出乳杆菌,进一步筛选出产生过氧化氢的优势菌株,并运用分子生物学方法,对其进行基因型鉴定。并对优势菌进行初步的性能鉴定试验。结果:从18名体检女性阴道分泌物内分离纯化得到11株菌,检出率为61.1%。分别为嗜酸乳杆菌4株,格氏乳杆菌3株,詹氏乳杆菌2株,卷曲、唾液乳杆菌各1株。结论:运用传统的细菌学分离方法与分子生物学对分离的菌株进行了鉴定。其中一株为产生过氧化氢的卷曲乳杆菌,经过初步测试,该菌株具有良好的耐酸、耐高温及体外拮抗其他病原微生物的能力。

Isolation and identification of lactobacillus from female genital tract

Objective:Isolating lactobacillus from health female genital tract, for exploring probiotics to research compound preparation.Methods:Traditional morphology analysis was used to identify lactobacillus, and further screen hydrogen peroxide-producing lactobacillus.The molecular biology method was used to identify exactly and to examine predominant strain by performance test.Results:There were 11 strains of lactobacillus isolated and identified from 18 health examination women genital tract, the detection rate was 61.1%.According to the results of these experiment,11 strains were identified that 4 were lactobacillus acidophilus, 3 were lactobacillus gasseri, 2 were lactobacillus jensenii,1 was lactobacillus crispatus and another one was lactobacillus salivarius.Conclusion:Traditional biochemical identification and molecular biology methods could identify lactobacillus.Only lactobacillus crispatus is hydrogen peroxide-producing one. After the preliminary test, the strain is a strong acid-resistant and hot-resistant one, and is bacteriostasis in vitro testing.