血清及尿液中sFlt-1/PLGF比值的变化与子痫前期相关性研究

目的:研究子痫前期患者及正常对照组孕妇血清及尿液中胎盘生长因子(PLGF)与可溶性络氨酸激酶受体1(sFlt-1)水平变化,探讨血清及尿液中sFlt-1/PLGF比值的变化与子痫前期病情程度的关系,以及尿液中PLGF、sFlt-1水平与血清中PLGF、sFlt-1水平的相关性。方法:采用酶联免疫吸附法检测并比较25例轻度子痫前期患者、20例重度子痫前期患者及20例正常晚期妊娠妇女血清及尿液中sFlt-1/PLGF比值,并分析子痫前期患者及对照组孕妇尿液中sFlt-1、PLGF与血清中sFlt-1、PLGF水平的相关性。结果:对照组血清及尿液中sFlt-1/PLGF值低于轻度子痫前期组,轻度子痫前期组血清及尿液中sFlt-1/PLGF值低于重度子痫前期组,各组之间的差异有统计学意义(P<0.05)。尿液中PLGF、sFlt-1水平与血清中PL-GF、sFlt-1水平成正相关。结论:孕妇血清及尿液中sFlt-1/PLGF比值水平与子痫前期病情程度呈相关性,且尿液中PLGF、sFlt-1水平与血清中PLGF、sFlt-1水平成正相关,可以用尿液中sFlt-1/PLGF比值检测替代血清sFlt-1/PLGF比值预测子痫前期的发生。

Study on the correlation between changes of sFlt - 1/PLGF ratios in serum and urine and preeclampsia

Objective:To investigate the changes of placental growth factor(PLGF) and soluble tyrosine kinase receptor 1(sFlt-1) levels in serum and urine of patients with preeclampsia and normal pregnant women,explore the relationship between changes of sFlt-1/PLGF ratios in serum and urine and degree of preeclampsia,and the correlation between levels of PLGF and sFlt-1 in urine and levels of PLGF and sFlt-1 in serum was also analyzed.Methods:Enzyme linked immunoassay(ELISA) was used to detect and compare sFlt-1/PLGF ratios in serum and urine from 25 patients with mild preeclampsia,20 patients with severe preeclampsia and 20 normal pregnant women of late pregnancy(control group),analyze the correlation between levels of PLGF and sFlt-1 in urine and levels of PLGF and sFlt-1 in serum in preeclampsia group and control group.Results:sFlt-1/ PLGF ratios in serum and urine of control group were significantly lower than those of mild preeclampsia group,sFlt-1/PLGF ratios in serum and urine of mild preeclampsia group were significantly lower than those of severe preeclampsia group,there was significant difference among the three groups(P<0.05);there was a positive correlation between levels of PLGF and sFlt-1 in urine and levels of PLGF and sFlt-1 in serum.Conclusion:There is correlation between sFlt-1/PLGF ratios in urine and serum and degree of preeclampsia in pregnant women,and there is a positive correlation between levels of PLGF and sFlt-1 in urine and levels of PLGF and sFlt-1 in serum,detection of sFlt-1/PLGF ratio in serum may be replaced by detection of sFlt-1/PLGF ratio in urine in order to predict the occurrence of preeclampsia.