| 晶状体抗氧化能力指数的测定 |
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| 引用本文： | 江涛,郑洁静,赵亮,续洁琨,姚新生,栗原博.晶状体抗氧化能力指数的测定[J].中药药理与临床,2007,23(1):77-79. |
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| 作者姓名： | 江涛 郑洁静 赵亮 续洁琨 姚新生 栗原博 |
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| 作者单位： | 1. 暨南大学中药及天然药物研究所,广州,510632
2. 沈阳药科大学中药学院,沈阳,110016 |
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| 摘 要： | 目的:建立晶状体抗氧化能力指数的测定方法。方法:以D-半乳糖诱导小鼠高血糖模型,采用ORAC法测定小鼠晶状体的抗氧化能力指数,HPLC法测定晶状体内谷胱甘肽及维生素C含量。结果:与正常组相比,D-半乳糖诱导高血糖模型组小鼠晶状体的抗氧化能力指数、谷胱甘肽及维生素C含量均显著性下降。结论:ORAC方法可用于测定晶状体的抗氧化能力指数,其活性与晶状体中谷胱甘肽及维生素C含量相关。
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| 关 键 词： | D-半乳糖 抗氧化能力指数 晶状体 谷胱甘肽 维生素C |
| 文章编号： | 24044664 |
Determination of oxygen radical absorbance capacity (ORAC) in lens |
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| Jiang Tao,Zheng Jiejing,Zhao Liang,Xu Jiekun,Yao Xinsheng,Hiroshi Kurihara.Determination of oxygen radical absorbance capacity (ORAC) in lens[J].Pharmacology and Clinics of Chinese Materia Medica,2007,23(1):77-79. |
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| Authors: | Jiang Tao Zheng Jiejing Zhao Liang Xu Jiekun Yao Xinsheng Hiroshi Kurihara |
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| Abstract: | Objective: To establish the oxygen radical absorbance capacity (ORAC) assay in assessing the total antioxidative capacity of lens and investigate its application. Methods: Hyperglycemic mice were treated with 30% D-galactose for 8 weeks. In addition, ORAC activity in lens was determined by automated multifunctional microplate reader. The contents of glutathione (GSH) and Vitamin C in lens were determined by high performance liquid chromatography (HPLC). Results: Compared with the normal group, ORAC activity and the contents of GSH and Vitamin C in lens of hyperglycemic mice decreased remarkably. Conclusion: ORAC assay may be applied to the assessment of total antioxidative capacity, and there is a good relationship between ORAC activity and the contents of GSH and Vitamin C in lens. |
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| Keywords: | oxygen radical absorbance capacity (ORAC) lens D-galactose glutathione (GSH) Vitamin C |
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