两个人食管癌细胞系的建立及染色体分析

目的结合传统细胞遗传学和新近发展起来的分子细胞遗传学方法,对１９８７年我室建立的两例食管癌细胞系ＥＣ８７１２和ＥＣ８７３３进行细胞遗传学分析。方法采用组织块培养法,用１．５％牛血清的１９９培养液,成功地建立了这两个细胞系,做了初步的生物学鉴定,并采用染色体Ｇ显带、染色体荧光原位杂交和比较基因组杂交等方法进行了细胞遗传学和分子遗传学的检测。结果发现两个细胞系经异种接种均形成移植性肿瘤,染色体为非整倍体,他们均有Ｙ染色体丢失、１ｐ缺失、２ｑ易位、５ｐ扩增,ＥＣ８７３３有８ｑ、１３ｑ扩增,ＥＣ８７１２有１７ｐ缺失。结论提示检出的这些畸变可能与肿瘤的发生、发展有关。

Establishment of two human esophageal carcinoma cell lines and their cytogenetic analysis

Objective To explore the relation between chromosomal aberration and carcinogenesis of the esophagus by molecular cytogenetic analysis of two esophageal carcinoma cell lines. Methods Small tissue blocks taken from resected specimens of patients with esophageal cancer (EC) were cultured in medium 199 added with 1.5% fetal bovine serun, and two esophageal carcinoma cell lines, EC8712 and EC8733, were established in 1987. They grew in monolayers. Molecular cytogenetic studies were performed on the two cell lines using chromosome G banding, fluorescence in situ hybridization (FISH) and comparative gene hybridization (CGH) techniques. Results Both cell lines were aneuploid. Loss of chromosome Y, partial deletion of 1p, translocation of 2q and amplification of 5p, were observed in the 2 cell lines. Amplification of 8q and 13q in EC8733 and deletion of 17p in EC8712 were detected. Conclusion The presene of the reported chromosomal aberrations may be related to the carcinogenesis and cancer development of the esophagus.