高淀粉膳食诱导高甘油三酯血症大鼠血浆及肝脏磷脂含量变化的研究

目的 研究高淀粉膳食诱导高甘油三酯血症(HTG)大鼠血浆及肝脏磷脂 (PL) 含量的变化.方法 实验组大鼠喂饲高淀粉膳食,6 d后氧化酶法测定血浆甘油三酯(TG)、胆固醇(TC)、血糖,抗坏血酸还原法测定PL含量.磷钨酸沉淀法分离高密度脂蛋白(HDL),测定HDL-TG、HDL-C、HDL-PL,计算NHDL-TG、NHDL-C和NHDL-PL浓度.结果 实验组大鼠较普通膳食喂饲的对照组血糖、TG、NHDL-TG、PL升高(P＜0.05),血浆HDL-C、HDL-PL浓度和HDL-PL/HDL-C比值降低(P＜0.05).实验组大鼠肝脏TG、PL含量升高(P＜0.05).相关性分析发现,实验组血浆PL与TG呈正相关,与HDL-PL呈负相关,与NHDL-TG正相关性较对照组增强(P＜0.05);实验组HDL-PL与血糖、血浆PL、肝脏PL呈负相关,与TG 、NHDL-TG负相关性较对照组增强(P＜0.05);实验组肝脏PL与肝脏TG相关性较对照组增强(P＜0.05).结论 高淀粉膳食诱导HTG模型大鼠伴血浆及肝脏PL含量增加,HDL中PL含量减少,血浆及肝脏PL含量与其他脂质含量的相关性状态也发生改变.

A Study on the Changes in Phospholipids Content of Plasma and Liver in Rats with Hypertriglyceridemia Induced by High-Starch Diet

Objective To investigate the changes of phospholipids content in plasma and liver in rats' hypertriglyceridemia(HTG) induced by high-starch diet,and to gain an insight into the relationship of triglyceride with phospholipids.Methods HTG rats were fed with high-starch diet,and the control rats with regular diet.After 6 days,triglyceride(TG),total cholesterol(TC),and blood glucose were measured by enzymatic kits.Phospholipids(PL) were measured by antiscorbutate reduction method.High density lipoproteins(HDLs) were separated by phosphotungistic acid.HDL-TG and HDLC were determined by enzymatic kits,HDL-PL contents were measured by antiscorbutate reduction method.NHDL-TG,NHDL-C and NHDL-PL contents were calculated by minus of HDL contents from total contents.TG,TC and PL in liver were measured by chemical assays.Results The fasting plasma TG,NHDL-TG,glucose and PL contents of rats fed by high-starch dietary were higher than those of the rats fed by regular diet.HDL-C,HDL-PL and HDL-PL/HDL-C ratio of HTG rats were lower than those of control rats.TG and PL in liver of HTG rats were higher than those of control rats.Correlation analyses indicated that the plasma PL of HTG rats had positive correlation with TG,negative correlation with HDL-PL,and stronger correlation with NHDL-TG when compared with that of control rats.In HTG rats,HDL-PL had negative correlation with glucose,PL,and liver PL.Furthermore,HDL-PL in HTG rats had stronger negative correlation with plasma TG and NHDL-TG as compared with those in control rats.PL in the liver of HTG rats had stronger correlations with TG in liver,compared with PL in the liver of control rats.Conclusion The rats with hypertriglyceridemia induced by high-starch diet have higher PL content in plasm and liver,but lower PL content in HDL,and their correlation pattern of PL in plasm and liver with other lipids is also changed.