| 表没食子儿茶素没食子酸酯对大鼠脊髓损伤后神经细胞凋亡及Caspase-3表达的影响 |
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| 引用本文： | 刘新宁,刁尧,兰振和,马婧,葛瑞,焦英,朱悦.表没食子儿茶素没食子酸酯对大鼠脊髓损伤后神经细胞凋亡及Caspase-3表达的影响[J].解剖学报,2014,45(1):20-25. |
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| 作者姓名： | 刘新宁 刁尧 兰振和 马婧 葛瑞 焦英 朱悦 |
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| 作者单位： | 1.中国医科大学实验技术中心，沈阳 110001; 2.阜新市第二人民医院神经内科，辽宁 阜新 123000; 3.中国医科大学附属第一医院骨科，沈阳 1100015. |
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| 基金项目： | 辽宁省自然科学基金资助项目(项目编号：20092118) |
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| 摘 要： | 目的观察表没食子儿茶素没食子酸酯(EGCG)对脊髓损伤(SCI)大鼠神经元凋亡和凋亡调控蛋白Caspase-3时间、空间表达的影响,探讨其脊髓损伤保护机制。方法 SD大鼠75只,分为假手术组、SCI组、EGCG组(n=25只);钳夹法制作脊髓损伤模型,EGCG组和SCI组损伤后即刻和1h后腹腔注射EGCG(50 mg/kg)和等量生理盐水;用结晶紫(CV)染色法、TUNEL和免疫组织化学方法,观察SCI后6h、12h、24h、3d和7d时间范围内,从损伤中心到头端0~5.00mm空间范围内,EGCG对脊髓神经元存活、凋亡以及相关调控蛋白Caspase-3表达的影响。结果 CV染色发现,SCI后6h~7d,在0~0.50mm空间范围内均未发现存活的神经元,在1.00~4.60mm范围内,EGCG和SCI组相比存活的神经元数明显增加(P0.01);TUNEL结果发现,SCI后6h~7d,在1.05~3.05mm范围内,EGCG与SCI组相比凋亡神经元数明显减少(P0.01);免疫组织化学发现,SCI后6h~7d,在1.10~3.10mm范围内,EGCG与SCI组相比Caspase-3阳性表达的神经元数明显减少(P0.01)。结论 EGCG在一定时间、空间范围内能下调Caspase-3表达,减少神经元凋亡,对继发性SCI有拮抗作用。
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| 关 键 词： | 脊髓损伤 表没食子儿茶素没食子酸酯 凋亡 Caspase-3 原位缺口末端标记 大鼠 |
| 收稿时间： | 2013-01-10 |
Effect of epigallocatechin gallate on temporal and spatial profiles of neurons apoptosis and Caspase-3 protein expression after spinal cord injury in rats |
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| LIU Xin-ning DIAO Yao LAN Zhen-he MA Jing GE Rui JIAO Ying ZHU Yue.Effect of epigallocatechin gallate on temporal and spatial profiles of neurons apoptosis and Caspase-3 protein expression after spinal cord injury in rats[J].Acta Anatomica Sinica,2014,45(1):20-25. |
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| Authors: | LIU Xin-ning DIAO Yao LAN Zhen-he MA Jing GE Rui JIAO Ying ZHU Yue |
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| Institution: | 1. Center of Experimental and Technology, China Medical University, Shenyang 110001,China; 2. Department of Neurology,the Second People’s Hospital, Liaoning Fuxin 123000,China; 3. Department of Orthopedics, the First Affiliated Hospital, ;
China Medical University, Shenyang 110001, China) |
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| Abstract: | Objective To evaluate the effect of epigallocatechin gallate(EGCG) on temporal and spatial profiles of neuron apoptosis and its associated Caaspase-3 protein expression after spinal cord injury in rats. Methods SD rats were randomly divided into three groups (25 rats each): sham-operated group, SCI group (normal saline solution intraperitoneal injection, immediately and 1 hour after SCI), and EGCG treatment groups (50mg/kg, intraperitoneal injection, immediately and 1 hour after SCI ). SCI model was made by clamps methods. The rats were sacrificed at 6, 12, 24 hours, 3 and 7days; 5 rats at each time point after injury. Spinal cord tissue in the injured area was harvested, sectioned 0-5.00 mm rostral from the epicenter post-injury and examined by cresyl violet (CV) staining and terminal-deoxynucleoitidyl transferase (TdT)-mediated deoxy uridine triphosphate-biotin nick end labeling (TUNEL) staining method and immunohistochemical method. Results At all the time points observed, CV-staining result showed that the neurons was not found in section 0-0.50 mm for SCI and EGCG group, the neurons was increased in EGCG group than in the SCI group in the sections 1.00-4.60 mm. The number of TUNEL positive neurons and Caspase-3 neurons were decreased in EGCG group than in SCI group in the section 1.05-3.05 mm and 1.10-3.10 mm rostral from the epicenter. Conclusion EGCG can decrease the expression of Caspase-3 and inhibit neuronal cell apoptosis in the rats after spinal cord injury on temporal and spatial profiles, which may play a significant neuroprotective role in the secondary SCI. |
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| Keywords: | Spinal cord injury Epigallocatechin gallate Apoptosis Caspase-3 TUNEL Rat |
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