直流小电场诱导乳腺癌细胞p21、p16基因表达与凋亡的初步研究

目的观察直流小电场对乳腺癌细胞p21、p16基因mRNA表达的诱导作用及与凋亡的关系.方法应用体外直流小电场作用于乳腺癌细胞系MCF7,观察其对乳腺癌细胞重要基因p21、p16表达以及凋亡的影响.结果直流小电场对乳腺癌细胞有明显的凋亡诱导作用,200 mV/mm组癌细胞大量脱落;电场组凋亡肿瘤细胞数随电场强度升高而增多(均为 P<0.01),p21、p16基因mRNA表达明显升高.结论直流小电场对乳腺癌细胞具有明显的凋亡诱导作用,可能与p21、p16基因表达相关.

Apoptosis of breast cancer cell line induced by small direct-current electric field and its related mechanism in expression of p21 and p16 genes

ObjectiveTo observe the apoptosis of breast cancer cells induced by small direct-current electric field (EF) and its related possible mechanism in expression of p21 and p16 genes.MethodsSmall direct-current EF was applied in human breast cancer cell line MCF7 in vitro and the effect of EF on apoptosis and expression of p21 and p16 important genes of breast cancer cells was observed.ResultsThe small direct-current EF had an obvious apoptosis inducing effect on breast cancer cells. A lot of cancer cells were sloughed from culture dish by 200 mV/mm of the EF strength. The number of the apoptotic cancer cells was gradually significantly increased and mRNA expressions of p21 and p16 genes were raised following the EF strength being up ( P<0.01).ConclusionThe small direct-current EF has an obvious apoptosis inducing effect on breast cancer cells. It may be related with the expression of p21 and p16 genes.