| 非胰岛素依赖型糖尿病与胰岛素基因实验研究 |
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| 引用本文： | 马灵筠,康健,余燕妮,陈群利,李建端,赵勤. 非胰岛素依赖型糖尿病与胰岛素基因实验研究[J]. 河南科技大学学报(医学版), 1997, 0(1) |
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| 作者姓名： | 马灵筠 康健 余燕妮 陈群利 李建端 赵勤 |
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| 作者单位： | 河南医科大学生化教研室，河南医科大学生化教研室 |
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| 摘 要： | 从非胰岛素依赖型糖尿病（NIDDM）患者外周血的白细胞中提取DNA．以胰岛素基因5’－末端上游的某一DNA序列为引物，对特定的靶序列进行体外扩增，即TaqDNA聚合酶链反应（PCR），将其扩增产物进行电泳分离，在紫外分析仪下观察结果并与正常人对照，发现16例NIDDM的M患者胰岛素基因有所改变，提示此型糖尿病患者的胰岛素基因改变可能是导致NIDDM遗传因素之一。
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| 关 键 词： | 非胰岛素依赖型糖尿病 胰岛素基因 多基因遗传病 |
The experimental studies of non-insulin-dependent diabetes Mellitus (NIDDM) and insulin gene |
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| Ma Lingjun, Kang Jian, Yu Yanni, Chen Qunli. The experimental studies of non-insulin-dependent diabetes Mellitus (NIDDM) and insulin gene[J]. Journal of Henan University of Science & Technology:Medical Science, 1997, 0(1) |
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| Authors: | Ma Lingjun Kang Jian Yu Yanni Chen Qunli |
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| Abstract: | DNA was extracted from the white blood cells of the patients with NIDDM,using some DNA sequence of insulin gene 5' -end upstream as primer amplifying the sequence of special target,that is,Taq DNA polymerase chain reaction (PCR). Electrophoretic segregation took place in the amplified substances. The result shows that under the ultraviolet lamp almost 16 patients with NIDDM represented abnormal straps. The changes of insulin gene in the pa-tients with this type of diabetes may result in one of NIDDM hereditary factors. |
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| Keywords: | insulin gene non-insulin-dependent diabetes mellitus (NIDDM ) multigenerational hereditary disease |
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