| 顺铂结合CIK细胞对裸鼠人胃癌模型体内的抗肿瘤作用 |
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| 引用本文： | 孙利兵,杨光.顺铂结合CIK细胞对裸鼠人胃癌模型体内的抗肿瘤作用[J].世界华人消化杂志,2010,18(12). |
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| 作者姓名： | 孙利兵 杨光 |
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| 作者单位： | 南京医科大学附属苏州医院肿瘤内科,江苏省苏州市,215001 |
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| 基金项目： | 苏州科技局基金资助项目 |
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| 摘 要： | 目的:探讨顺铂、CIK细胞以及顺铂联合CIK对裸鼠人胃癌移植瘤生长的抑制作用,为临床上联合应用顺铂和CIK细胞治疗胃癌提供实验依据.方法:应用淋巴细胞分离液分离外周血单个核细胞,给予多种细胞因子(rhIFN-g、CD3mcAb、rh1L-1、rhlL-2),诱导生成CIK细胞;培养人胃癌细胞株SGC-7901,接种至80只裸鼠右腋皮下,10 d后取移植瘤直径基本一致的裸鼠随机分4组:NS对照组、顺铂组、CIK细胞组和顺铂+CIK细胞组,每组16只.连续5 d在接种肿瘤细胞部位处给予相应注射治疗,观察其对胃癌移植瘤模型的抗肿瘤疗效.结果:与NS对照组相比,顺铂组、CIK细胞组、顺铂+CIK细胞组裸鼠人胃癌移植瘤模型治疗后肿块质量均减轻,生存期均明显延长,尤以顺铂+CIK组效果显著(P<0.01).裸鼠体内实验表明,顺铂+CIK细胞联合应用能够显著抑制胃癌细胞的生长,其抑瘤率可达57.8%,明显高于NS对照组(P<0.01).免疫功能检测显示红细胞C3b反应受体明显升高,红细胞免疫复合物受体明显降低(P<0.01).结论:顺铂联合CIK细胞对人胃癌移植瘤生长的抑制作用大于单独应用顺铂或CIK细胞.
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| 关 键 词： | 细胞因子诱导的杀伤细胞 顺铂 胃癌 裸鼠 |
Antitumor effects of cisplatin and CIK cells against human gastric cancer in nude mice |
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| Li-Bing Sun,Guang Yang.Antitumor effects of cisplatin and CIK cells against human gastric cancer in nude mice[J].World Chinese Journal of Digestology,2010,18(12). |
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| Authors: | Li-Bing Sun Guang Yang |
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| Institution: | Li-Bing Sun,Guang Yang,Department of Oncology,Suzhou Municipal Hospital Affiliated to Nanjing Medical University,Suzhou 215001,Jiangsu Province,China |
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| Abstract: | AIM:To investigate the antitumor effects of cisplatin and cytokine-induced killer (CIK) cells,alone and in combination,against human gastric cancer in nude mice.METHODS:Peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers by Ficoll gradient centrifugation.CIK cells were induced by culturing PBMCs in the complete medium containing recombinant human interferon-g (rhIFN-g),anti-CD3 monoclonal antibody (mcAb),recombinant human interleukin-2 (rhlL-2),and recombinant human interleukin-... |
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| Keywords: | Cytokine-induced killer cell Cisplatin Gastric cancer Nude mouse |
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