流式细胞仪检测缺氧/缺血神经细胞凋亡的研究

目的：研究人神经母细胞瘤细胞株(SII—SY5Y)缺氧／缺血后凋亡细胞的百分率及细胞周期的变化。方法：用流式细胞检测技术，用培养的连续细胞系细胞株，建立缺氧／缺血诱导的神经细胞凋亡模型，观察随缺氧程度的加重，凋亡细胞百分率的变化。结果：细胞缺氧／缺血培养8h可诱导细胞凋亡。流式细胞仪检测结果显示：在G1峰前出现一个G1亚峰，为凋亡峰(Ap峰)，随缺氧程度的加重，其凋亡比率递增。细胞周期分析则显示S及G2期逐渐下降，与凋亡的百分率呈负相关。结论：缺氧／缺血损伤可致培养的神经细胞凋亡，凋亡比率与缺氧程度呈正相关。缺氧缺血损伤可引起细胞周期的变化，G1亚峰峰值与S、G2期的峰值呈负相关。提示：缺氧／缺血损伤引起细胞凋亡过程中，细胞增殖及分裂处于抑制状态；凋亡是神经细胞缺氧／缺血损伤的重要死亡形式。

Model SH-SY5Y cells apoptosis induced by hypoxia/hypoglycemia

Purpose\ To study change of neuron apoptosis induced and cell cycle by hypoxia/hypoglycemia on SH\|SY5Y. Method\ Model of neuron hypoxia/hypoglycemia were established by sodium peroxydisulfate treatment and glucose deprivation on cultured SH\|SY5Y cells.Apoptosis assayed by flow cytometry method.To observe anoxia extent increased and apoptosis was changed.Result\ Hypoxia/hypoglycemia of SH\|SY5Y cell cultures for 8h to induce typical apoptotic changes.FCM detection:before G1 appeard Ap second peak.Cell cycle showed S and G2 period were lower.Conclusions\ Hypoxia/hypoglycemia induced apoptosis and changed cell cycle in SH\|SY5Y cells.Apoptosis was positively correlated with anoxia.Ap second peak was negative correlated with S and G2 period.Hypoxia/hypoglycemia induced apoptosis,cellproliferation and split in SH\|SY5Y cells were in restrained state.apoptosis was one of the death pattern at nerve cells.