急性内毒素血症早期大鼠淋巴管运动变化及其一氧化氮合酶组织学研究

目的:研究急性内毒素血症早期大鼠淋巴管运动变化和一氧化氮合酶(NOS)的相关组织染色,探讨二者的关系。方法:股静脉注脂多糖(LPS),观察大鼠肠系膜淋巴管2h内的运动变化及相关组织的酶组化、HE染色。结果:LPS组淋巴管管径变大,运动频率减少,镜下见小肠绒毛中央乳糜管及粘膜下淋巴管丛极度扩张;对照及LPS组淋巴管内皮细胞及管壁上神经纤维都含有NOS阳性反应产物。结论:内毒素血症早期淋巴管扩张,运动减少,NOS可能在其中起重要作用。

Histochemical study on thechange in lymphatic motion and nitric oxide synthase in the early phase of acute ratendotoxemia

AIM:To study the changes in lymphatic motion and histochemical stain of nitric oxide synthase(NOS) in the early phase of acute rat endotoxemia and explore their relationship. METHODS: After lipopolysaccharide (LPS) was injected into femoral vein, rats' mesenteric lymphatic were inspected within two hours. Paraffin section was stained by HE, frozen section by NADPH diaphorase histochemistry. RESULTS: In LPS group, the mesenteric lymphatic diameter was greater than that in the control and contractile frequency was decreased, the villi central lacteal and submucosal microlymphatics of small intestine was extremely dilated under microscope. In LPS and control group, NOS was positive in lymphatic endothelia and the nerve fibers in the vessels' wall. CONCLUSION: In the early stage of acute endotoxemia, the lymphatic vessles dilated and contractile activity diminished, NOS may play an important role in these changes.