| 抗-HCV不同试剂检测结果分析 |
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| 引用本文： | 胡京辉,张磊,孙莉. 抗-HCV不同试剂检测结果分析[J]. 军医进修学院学报, 2009, 30(4): 498-499 |
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| 作者姓名： | 胡京辉 张磊 孙莉 |
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| 作者单位： | 北京市血液中心,100088 |
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| 摘 要： | 目的探讨不同检测方法、试剂对丙型肝炎病毒（HCV）抗体检测结果的影响。方法以不同试剂采用酶联免疫吸附试验（ELISA）对献血者血浆标本进行抗-HCV检测，呈反应性标本采用HCV RNA RT—PCR荧光定量确证检测。结果ELISA再次检测抗HCV抗体阳性率明显低于初次检测阳性率，差异有统计学意义（P〈0．01），确证试验阳性率低于初次检测和再次检测的阳性率，差异有统计学意义（P〈0．05）。结论ELISA测定结果为HCV抗体阳性者应经HCVRNART—PCR荧光定量加以确认。
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| 关 键 词： | 肝炎,丙型,慢性 肝炎抗体,丙型 酶联免疫吸附测定 |
Detection of anti-HCV antibody with different methods |
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| HU Jing-hui,ZHANG Lei,SUN Li. Detection of anti-HCV antibody with different methods[J]. Academic Journal of Pla Postgraduate Medical School, 2009, 30(4): 498-499 |
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| Authors: | HU Jing-hui ZHANG Lei SUN Li |
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| Affiliation: | HU Jing-hui,ZHANG Lei,SUN Li Department of Clinical Laboratory,Beijing Red Cross Blood Center,Beijing 100088,China |
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| Abstract: | Objective To investigate the impact of different methods and reagents on detection of anti-HCV antibody in blood donors. Methods Anti-HCV antibody in blood donors was detected by enzyme-linked immunosorbent assay(ELISA)using different reagents,and positive sera were examined by Western blotting confirmatory test.Results Western blotting confirmatory test showed a lower positive anti-HCV rate than ELISA(P<0.05).Conclusion Positive HCV antibody detected by ELISA should be confirmed by quantifying fluorescence... |
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| Keywords: | hepatitis C chronic hepatitis C antibodies enzyme-linked immunosorbent assay |
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