A comparison of antibody capture radio- and enzyme immunoassays with immunofluorescence for detecting IgM antibody in infants with congenital rubella |
| |
Authors: | Shireen Chantler C.J. Evans P.P. Mortimer J.E. Cradock-Watson Margaret K.S. Ridehalgh |
| |
Affiliation: | 1. Wellcome Research Laboratories, Beckenham, Kent, BR3 3BS, U.K.;2. Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London, NW9 5HT, U.K.;3. Public Health Laboratory, Withington Hospital, Manchester M20 8LR, U.K. |
| |
Abstract: | IgM antibody capture radioimmunoassay (MACRIA) and enzyme immunoassay (MACEIA) were compared with immunofluorescence (IF) for detecting specific IgM antibody in 99 sera from 76 infants with confirmed congenital rubella, and 61 sera from a comparative group of 59 infants who had miscellaneous abnormalities but in whom congenital rubella was not confirmed.All of 35 specimens collected from confirmed cases within 12 weeks of birth were positive by all three methods and all but one of 17 specimens collected after the age of 18 months were uniformly negative. At intermediate ages discrepancies occurred in 18 specimens, of which eight were positive and 10 negative by IF. Three of these 18 specimens were negative by both antibody capture procedures but showed weak fluorescence; the other 15 were negative by MACEIA, but positive by MACRIA which appears to be the more sensitive of the antibody capture methods. Sera from five infants in the comparative group were clearly positive by all three methods. These five infants were probably congenitally infected with rubella. Sera from the other 54 infants were negative, except for one that gave a weakly positive result by MACRIA alone.Antibody capture procedures offer several advantages over previous methods for detecting IgM antibody. Although MACRIA was found to be slightly more sensitive than MACEIA, the greater stability of the enzyme label, together with the possibility of both visual and quantitative assessment, could make MACEIA the method of choice for detecting rubella-specific IgM. |
| |
Keywords: | rubella IgM antibody enzyme immunoassay radioimmunoassay |
本文献已被 ScienceDirect 等数据库收录! |
|