喉鳞癌组织中Bcl—2和P53蛋白表达及意义

为了探讨ｂｃｌ－２，Ｐ５３在喉鳞癌组织中的表达情况，预后的意义，我们应用免疫组化ＬＳＡＢ法对５３例喉鳞状上皮细胞癌组织进行ｂｃｌ－２，Ｐ５３基因蛋白表达的测定。结果显示：（１）４７．１７％的喉癌中检出突变型Ｐ５３蛋白，在部分喉癌旁不典型增生上皮中可见Ｐ５３蛋白表达，癌旁组织细胞已发生Ｐ５３基因突变可能是术后复发的根源，突变型Ｐ５３蛋白过度表达是喉癌发生的早期事件。

THE EXPRESSION OF BCL-2 P53 PROTEIN IN LARYNGEAL SQUMAOUS CELL CARCINOMA AND ITS SIGNIFICANCE

In order to study the expression of bcl- 2, p53 protein in laryngeal squmaous cell carcinoma(LSCC), the prognostic significance, their expression in 53 cases with laryngeal squmaous cell carcinoma were determinded by immunohistochemical LSAB method. The results showed that:(1)In 47.17%LSCC, the expression of mutant p53 protein were positive. Meanwhile, mutant p53 protein was also expressed in some dysplastic epitheliumes near the cancer tissues. These epithelial cells near the tumor in which p53 gene has been altered, probably are the source of tumor recurrence, the expression of mutant p53 protein may be the early events in the development of LSCC. The p53 protein expression was associated with low histological grade of differentiation, high mitotic count((P<0.05,P<0.01). The necrosis in tumors with the p53 positive was higer than those with the P53 negative LSCC(P<0.01),patients with p53 -positive LSCC had shorter survival times than patients with p53-negative LSCC.p53 protein expression was not associated with node status and age, is valuable in determinding the degree of malignancy, predicting tumor differentiation (2)In LSCCs, bcl- 2 expression was consistently low and related with low mitotic count and younge age(P<0.05,P<0.05),and bcl-2 expression was not related with histological grade of differentiation, necrosis, node status and survival time.