Photolytic Labeling and Its Applications in Protein Drug Discovery and Development |
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Authors: | Yuan Chen Elizabeth M. Topp |
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Affiliation: | Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, West Lafayette, Indiana 47907 |
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Abstract: | In this mini-review, the major types of photolytic labeling reagents are presented together with their reaction mechanisms. The applications of photolytic labeling in protein drug discovery and development are then discussed; these have expanded from studies of protein-protein interactions in vivo to protein-matrix interactions in lyophilized solids. The mini-review concludes with recommendations for further development of the approach, which include the need for new and more chemically diverse photo-reactive reagents and better understanding of the mechanisms of photolytic labeling reactions in various media. |
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Keywords: | photolytic labeling protein drug phenyl azide carbene diazirine benzophenone protein-protein interaction lyophilized solid |
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