1.Department of Dermatology,2nd Affiliated Hospital of Dalian Medical University,Dalian, Liaoning Province,P. R. China;2.College of Integrative Medicine,2nd Affiliated Hospital of Dalian Medical University,Dalian, Liaoning Province,P. R. China;3.Department of Clinical Laboratory,2nd Affiliated Hospital of Dalian Medical University,Dalian, Liaoning Province,P. R. China;4.Department of Dermatology,Northern California Institute for Research and Education,San Francisco,USA
Abstract:
Background
Recent studies have shown that vitiligo is a T-cell mediated autoimmune disease. Skin-homing cytotoxic T lymphocytes expressing cutaneous lymphocyte-associated antigen (CLA) have been suggested to be responsible for the destruction of melanocytes in vitiligo. An aberration in the suppressive function of regulatory T cells (Tregs) has been reported in vitiligo patients. However, whether the weakened suppressive ability of the Tregs contributes to hyper-activated skin homing CD8+CLA+ T cells remains to be determined.
Objectives
To investigate the inhibition of circulating Tregs on the proliferation of autologous CD8+CLA+ T cells in non-segmental vitiligo patients.
Methods
CD8+CLA+ T cells and Tregs were obtained from the peripheral blood of 13 non-segmental vitiligo patients and 7 controls. The proliferative responses of CD8+CLA+ T cells were assessed in the absence or presence of autologous Tregs, and the levels of Transforming Growth Factor β1(TGF-β1) and IL-10 in culture supernatants were detected by enzyme-linked immunosorbent assay.
Results
The proliferative responses of circulating CD8+CLA+ T cells in the presence of Tregs were significantly higher in the active vitiligo than in the stable vitiligo and control groups. Tregs from active vitiligo patients exhibited a lower inhibitory effect on proliferation of CD8+CLA+ T cells. The levels of TGF-β1 produced by Tregs were significantly lower in active vitiligo than other groups and anti-TGF-β1 antibodies could abrogate the suppressive function of Tregs.
Conclusions
The functional activity of Tregs is compromised in active vitiligo patients. TGF-β1 plays an important role in the autoimmune mechanism of the disease.