| 紫外可见分光光度法测定柴胡中柴胡总皂苷的含量 |
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| 引用本文： | 浦锦宝,胡轶娟,梁卫青,郑军献,魏克民. 紫外可见分光光度法测定柴胡中柴胡总皂苷的含量[J]. 医学研究杂志, 2008, 37(6): 98-100 |
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| 作者姓名： | 浦锦宝 胡轶娟 梁卫青 郑军献 魏克民 |
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| 作者单位： | 浙江省中医药研究院,杭州,310007 |
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| 摘 要： | 目的 建立柴胡药材中总皂苷的含量测定方法.方法 采用紫外可见分光光度法,以柴胡皂苷d为对照品,在波长536nm处对样品中的总皂苷进行含量测定.结果 柴胡总皂苷在194～1940 mg/L有良好的线性关系(r=0.9996),该法的平均加样回收率为97.35%,RSD为1.02%(n=9).结论 所建立的方法准确、简便、快速,重现性好,适用于柴胡中总皂苷的含量测定.
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| 关 键 词： | 紫外可见分光光度法 柴胡 总皂苷 含量测定 紫外可见分光光度法 分光光度法测定 柴胡总皂苷 含量测定方法 Bupleurum Saikosaponins Assay 快速 平均加样回收率 线性关系 结果 样品 波长 对照品 柴胡皂苷 柴胡药材 |
UV-Visible Spectrophotometric Assay for Saikosaponins in Bupleurum Chinense DC |
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| Pu Jinbao,Hu Yijuan,Liang Weiqing,Zheng Junxian,Wei Kemin. UV-Visible Spectrophotometric Assay for Saikosaponins in Bupleurum Chinense DC[J]. Journal of Medical Research, 2008, 37(6): 98-100 |
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| Authors: | Pu Jinbao Hu Yijuan Liang Weiqing Zheng Junxian Wei Kemin |
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| Affiliation: | Pu Jinbao,Hu Yijuan,Liang Weiqing,Zheng Junxian,Wei Kemin.Zhejiang Traditional Chinese Medicine Academy,Hangzhou 310007,China |
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| Abstract: | Objective To set up a simple and accurate method for the determination of saikosaponins in Bupleurum chinense DC. by UV-Visible Spectrophotometry. Methods The content of saikosaponins was determined by UV-Visible Spectrophotometry. The absorbency was measured at 536nm. Results The calibration curves was linear within 194~1940mg/L(r=0.9996). The average recovery was 97.35%,and the RSD was 1.02%(n=9). Conclusion The method was proved to be simple, precise and reproduciable. It can be used for the quantitative... |
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| Keywords: | UV-Visible spectrophotometry Bupleurum chinense DC. Saikosaponins Content determination |
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