加味四逆散药物血清对HSC-T6增殖的影响

目的: 观察加味四逆散含药血清对瘦素刺激肝星状细胞增殖的影响,探讨其抗肝纤维化的机制。方法: SD大鼠30只,随机分为3组:正常血清组、秋水仙碱对照组、加味四逆散组(37.5 g ·kg^-1),每组均10只,将正常血清组大鼠每只按10 mL ·kg^-1给予生理盐水ig,1次/日;秋水仙碱对照组大鼠每只按0.2 mg ·kg^-1给予秋水仙碱ig,1次/日;加味四逆散组给予加味四逆散药液10 mL ·kg^-1ig,1次/日。以上各组均连续ig 7 d,制备各药物含药血清,并将药物血清用培养基配制成5%,10%,20%,40% 4个浓度;培养肝星状细胞,取对数生长期细胞,使用噻唑蓝(MTT)比色法检测药物血清对100 mg ·L^-1的瘦素刺激肝星状细胞-T6(HSC-T6)增殖的影响。结果: 加味四逆散药物血清对瘦素刺激的肝星状细胞增殖有抑制作用(P<0.05或P<0.01), 在5%~40%的血清浓度范围内,抑制作用呈剂量依赖性关系;秋水仙碱对照组亦能抑制HSC-T6细胞的增殖(P<0.05),但在10%~40%的血清浓度范围内加味四逆散组对HSC-T6增殖的抑制率显著高于秋水仙碱对照组(P<0.05)。结论: 加味四逆散药物血清具有抑制瘦素刺激HSC-T6增殖的作用。

Effect of Jiawei Sini San Serum on Proliferation of HSC-T6

Objective:To observe effect of Jiawei Sini San(JWSNS) serum on proliferation of hepatic stellate cells(HSC-T6) and to investigate the mechanism of hepatic fibrosis. Method:Thirty rats were randomly divided into three groups: normal serum, colchicine group and Jiawei Sinisan group, 10 rats each group. The normal serum group was given saline, 10 mL ·kg^-1 ig, once each day; the colchicine group was given colchicine, 0.2 mg ·kg^-1 ig, once each day; JWSNS group was given liquid of JWSNS 10 mL ·kg^-1 ig, one each day. The three groups were administrated for 7 d. Drug serum was prepared with the medium to 5%, 10%, 20%, 40%; hepatic stellate cells was cultured, logarithmic growth phase of cells, was assayed by MTT for measuring serum leptin stimulation of 100 ng ·mL^-1 HSC-T6 proliferation. Result: JWSNS serum inhibited the proliferation of hepatic stellate cells (P<0.05 or P<0.01) with,a dose-dependent manner; colchicine control group also inhibited the proliferation of HSC-T6 cells (P<0.05), but inhibition rate of 10% to 40%drug serum was significantly higher than that of colchicine group (P<0.05). Conclusion: JWSNS has the suppression on the proliferation of HSC-T6.