GC-MS法分析中药索骨丹中脂肪酸成分

目的: 分析秦岭南坡中药材索骨丹中脂肪酸成分。方法: 采用乙醚索氏抽提法提取中药材索骨丹中的脂肪油,经BF₃-CH₃-OH甲酯化处理,用气相色谱-质谱连用法(GC-MS)测定脂肪酸的组成。结果: 索骨丹的脂肪酸含量为1.686%,试验共分出24个峰,确定了索骨丹所含有的24种脂肪酸成分主要为亚油酸(41.539%)、α-亚麻酸(23.191%)、棕榈酸(18.824%)、硬脂酸(3.408%)、芥酸(1.506%)、油酸(1.388%)和二十四烯酸(1.036%),不饱和脂肪酸总含量高达70.314%。结论: 秦岭南坡产索骨丹药材中含有多种重要脂肪酸成分,结果为进一步完善索骨丹的系统研究提供了科学依据。

Analysis Fatty Acids Components in Rhizome of Rodgersia aesculifolia by GC-MS

Objective: The fatty acid components from the rhizome of Rodgersia aesculifolia which were produced in Liuba of the Qinling-Daba mountains in Shaanxi province. Method: The contents and chemical compositions of the fatty acids in rhizome of R. aesculifolia have been investigated and determined in this paper. Fatty oil was extracted with petroleum ether. The oil was determined by GC-MS after the preparation of the methyl esters with BF₃-CH₃-OH. Result: The experimental results showed that there were 24 components of fatty acids in the oil of R. aesculifolia by GC-MS. There are linoleic acid(41.539%),α-linolenic acid(23.191%), pamitic acid(18.824%), stearic acid(3.408%), erucic acid(1.506%), oleic acid(1.388%) and nervonic acid(1.036%). The unsaturated fatty acids accounted for 70.314%. Conclusion: The exepriment reveals were some impotent fatty acids in R. aesculifolia. These results provide theoretical basis for chemical constituent research of R. aesculifolia and future extent research.