SPE-HPLC-ELSD法测定复方藏药制剂 肝纤消颗粒中黄芪甲苷的含量

目的: 建立SPE-HPLC-ELSD法测定复方藏药制剂肝纤消颗粒中黄芪甲苷含量的方法。方法: 采用固相萃取系统对肝纤消颗粒进行处理,色谱柱为Alltima C₁₈ (4.6 mm×250 mm,5 μm),流动相乙腈-水 (33∶67),流速1.0 mL ·min^-1,柱温35 ℃,ELSD漂移管温度110 ℃,载气流量2.7 L ·min^-1,进样量 20 μL。结果: 黄芪甲苷在1.014~20.280 μg峰面积积分值自然对数与进样量自然对数有良好的线性关系线,r=0.999 3;平均加样回收率为100.1%,RSD 2.6%。结论: 该方法操作简单,准确,重复性好,可用于藏药肝纤消颗粒的质量控制。

Determination of Astragaloside in Tibetan Medicine Preparation Ganxianxiao Granules by SPE-HPLC-ELSD

Objective: To establish an analysis method to determine astragaloside in Tibetan medicine Preparation Ganxianxiao granules by SPE-HPLC-ELSD.Method: Samples were processed by the SPE,and determined on an Alltima C18 column(4.6 mm×250 mm,5 μm),the mobile phase consisted acetonitrile-water(33∶67) with flower rate at 1.0 mL·min-1 and column temperature at 35 ℃,while drift tube temperature was 110 ℃ of ELSD,by the carrier flow was 2.7 L·min-1,and injection volume was 20 μL.Result: The linear ranges of astragaloside was 1.014-20.280 μg(r=0.999 3),with the average recovery were of 100.1%(RSD 2.6%).Conclusion: The method is simple,accurate,reproducible and it can be used for the quality control of Tibetan medicine Preparation Ganxianxiao granules.