红花总黄酮大孔树脂纯化工艺

目的: 研究大孔树脂精制红花总黄酮的工艺条件。方法: 以红花总黄酮吸附率与解析率为指标,考察6种不同型号的大孔树脂对红花总黄酮的吸附与解析能力,确定AB-8为最佳树脂。对AB-8大孔树脂的纯化条件进行优化。结果: 最佳工艺条件为以50 g ·L^-1的质量浓度(药液pH 2.0)、4 BV ·h^-1的流速上样,药液最佳上样体积为3.3 BV(树脂量),以3 BV 30％乙醇2 BV ·h^-1的速率洗脱。经AB-8处理后的红花总黄酮纯度达54.04％,羟基红花黄色素A纯度达28.76％,分离效果良好。结论: AB-8型大孔吸附树脂能较好地纯化富集红花总黄酮。

Purification Technology of Total Flavonoids from Carthamus tinctorius by Macroporous Resin

Objective:To study on purification technology conditions of total flavonoids from Carthamus tinctorius by macroporous resin. Method: Absorption rate and desorption rate of total flavonoids were taken as indexes, investigated absorption and desorption ability of 6 different types macroporous resin to safflower flavonoids,determined AB-8 was optimum macroporous resin.Purification conditions of AB-8 macroporous resin were optimized. Result:Optimum technology conditions were as follows:liquid concentration was 50 g ·L^-1 (pH 2), flow rate of sample was 4 BV ·h^-1; eluted with 3 BV the amount of 30% ethanol by speed of 2 BV ·h^-1.After purified with AB-8 macroporons resin,purity of total flavonoids was up to 54.04%,purity of hydroxysafflor yellow A was up to 28.76%, separation effect was good. Conclusion: AB-8 resin was fit for separating and purifying of total flavonoids from safflower.