| 芦荟大黄素对T淋巴细胞增殖和细胞[Ca2+]i变化的影响 |
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| 引用本文： | 李臣宇,李新,刘丽军,邹洁,胡芬,李俊英.芦荟大黄素对T淋巴细胞增殖和细胞[Ca2+]i变化的影响[J].医学教育探索,2008(8). |
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| 作者姓名： | 李臣宇 李新 刘丽军 邹洁 胡芬 李俊英 |
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| 作者单位： | 南开大学生物物理系生物活性材料教育部重点实验室 |
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| 基金项目： | 天津市科委重点科技攻关项目 |
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| 摘 要： | 目的研究芦荟大黄素对植物血凝素(PHA)引起的大鼠T淋巴细胞增殖和细胞内Ca2 浓度(Ca2 ]i)变化的影响,探讨芦荟大黄素对T淋巴细胞增殖的作用和机制。方法采用MTT法检测细胞增殖,用单细胞钙成像系统记录细胞胞浆Ca2 ]i。结果芦荟大黄素对PHA引起的细胞增殖有显著抑制作用,且其抑制作用与剂量有关;加入PHA引起细胞Ca2 ]i迅速升高,并持续维持高Ca2 ]i水平,芦荟大黄素对PHA引起的Ca2 ]i升高有显著抑制作用,其作用途径包括阻断胞内Ca2 释放和抑制胞外Ca2 内流。结论芦荟大黄素可抑制T淋巴细胞增殖,其作用机制可能与抑制淋巴细胞内Ca2 ]i升高相关。
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| 关 键 词： | 芦荟大黄素 T淋巴细胞 细胞内游离Ca~(2 )浓度[Ca~(2 )]_i 增殖 |
Effect of aloe-emodin on proliferation and [Ca2+]i mobilization of T lymphocytes |
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| LI Chen-yu,LI Xin,LIU Li-jun,ZOU Jie,HU Fen,LI Jun-ying.Effect of aloe-emodin on proliferation and [Ca2+]i mobilization of T lymphocytes[J].Researches in Medical Education,2008(8). |
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| Authors: | LI Chen-yu LI Xin LIU Li-jun ZOU Jie HU Fen LI Jun-ying |
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| Abstract: | Objective To investigate the effects of aloe-emodin on PHA-induced proliferation and intracellular Ca2 mobilization of T lymphocytes.Methods The proliferation of T cell was tested by MTT assay.The Ca_(2 )]_i mobilization was recorded by single cell Ca2 image system using Fura-2 as Ca2 probe.Results Aloe-emodin inhibited PHA-induced proliferation in a dose-dependent manner.PHA triggered a rapid Ca_(2 )]_i rise followed by a sustain high Ca_(2 )]_i level.The rapid Ca2 rise was caused by Ca2 release from intracellular store and the sustain high i level was due to Ca2 influx.Aloe-emodin inhibited both Ca2 release and Ca2 influx.Conclusion Aloe-emodin blocks PHA-induced proliferation of T lymphocytes and the mechanism for this may be due to its inhibition on PHA-induced Ca_(2 )]_imobilization. |
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| Keywords: | aloe-emodin T lymphocyte intracellular Ca~(2 ) concentration proliferation |
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