天钩降压胶囊中丹皮酚在大鼠体内药代动力学

目的: 研究不同剂量天钩降压胶囊对大鼠体内丹皮酚药动学的影响。方法: 大鼠分别灌胃给予低、中、高剂量天钩降压胶囊(相当于丹皮酚6.884,13.77,27.53 mg ·kg^-1),于给药后不同时间采集大鼠血浆。血浆样品用甲醇沉淀蛋白,HPLC测定丹皮酚含量,各给药组平均血药浓度-时间数据采用DAS2.0软件分析。组间药动学参数用 SPSS 16.0 软件进行统计分析。结果: 低剂量组大鼠丹皮酚血药浓度在消除相超出最低检测限,未能测出药时曲线;中、高剂量组丹皮酚均在5 min达最大吸收;t1/2ka,Cmax,AUC_0-∞,AUC_0-360,CLz/F随给药剂量增加而显著性增加;t1/2z, MRT_0-360,Vz/F随给药剂量增加而显著性减小。结论: 大鼠灌胃不同剂量天钩降压胶囊后丹皮酚的药动学参数存在一定差异。

In vivo Pharmacokinetics of Paeonol from Tiangou Jiangya Capsule in Rats

Objective: To study on influence of in vivo pharmacokinetics of paeonol(Pae) from different dosages of Tiangou Jiangya capsule in rats.Method: Three dosages(low,medium,high) of TianGou Jiangya capsule(equivalent to paeonol 6.884,13.77,27.53 mg·kg-1) were fed to rats by intragastric administration separately.Plasma samples were collected at different time and treated with methanol to precipitate protein.The contents of paeonol in plasma were determined by HPLC.Pharmacokinetic parameters between groups were statistical analyzed by SPSS 16.0.Result: Plasma concentration of paeonol was beyond minimum detection limit and plasma concentration-time curve failed to detect at low dosage group;Maximum absorption of middle and high dosages of paeonol reached the maximum absorption in 5 minute,t1/2ka,Cmax,AUC0-∞,AUC0-360,CLz/F increased significantly with increasing of intragastric administration dosages.t1/2z,MRT0-360,Vz/F reduced Significantly with increasing of intragastric administration dosages.Conclusions:Pharmacokinetics parameters of paeonol had some differences after intragastric administration of different dosages of Tiangou Jiangya capsule.