余甘子总酚酸和总黄酮配伍抑制肝癌细胞增殖及 对免疫功能的调节作用

目的: 观察余甘子总酚酸和总黄酮不同配伍比例对癌细胞的抑制作用及观察对免疫系统的影响。方法: 在96孔培养板中,每孔加入100 μL (含有5 000个／mL肿瘤细胞) RPMI 1640培养液,采用MTT法观察不同配伍比例的余甘子总黄酮和总酚酸对人肝癌细胞株HepG2的体外抑制作用; 将64只小鼠随机分成8组,分别为正常对照组和余甘子总酚酸总黄酮配伍后的给药组,每天ig给药1次,连续9 d,采用MTT法测定小鼠脾淋巴细胞的转化增殖,测定小鼠血清白介素-2(IL-2)和干扰素-γ(IFN-γ)含量,测定不同配伍比例的余甘子总酚酸和总黄酮的免疫调节作用。结果: MTT法测定的结果均显示,不同比例余甘子总酚酸+总黄酮的配伍,对肝肿瘤细胞的抑制作用明显,其中以总黄酮比总酚酸10∶1(110 mg · L^-1∶11 mg · L^-1)时抑制率最高,抑制率为60.35%。余甘子总黄酮与总酚酸配伍能促进小鼠脾淋巴细胞增殖,其中每天以总酚酸329.5 mg ·kg^-1总黄酮228 mg ·kg^-1对小鼠ig给药组脾淋巴细胞增殖率最高,刺激指数比空白组高了0.786。并且促进小鼠淋巴细胞分泌因子的生成。结论: 余甘子总酚酸和总黄酮对肝脏肿瘤细胞增殖有明显的抑制作用,并且能增强机体特异性免疫功能和非特异性免疫功能的作用。

The Inhibition of Cancer Cell Proliferation and Regulating the Immune Function by Compatibility of Ratio Emblica Total Phenolic Acids and Flavonoids

Objective: To observe the inhibition of different compatibility ratio of emblica total phenolic acids and flavonoids on tumors and the effects on the immune system.Method: 100 μL(containing 5 000 cells / mL of tumor cells) RPMI 1640 culture medium was added to each well of 96-well plate,the MTT method was used to observe inhibition of human liver cell lines HepG2 by different compatibility ratio of emblica total phenolic acids and flavonoids in vitro,64 mice were randomly divided into eight groups,namely normal control group and the total phenolic flavonoids emblica compatibility treatment group,oral administration was performed,once dayly,lasting 9 days.MTT was used to measure proliferation of mouse spleen lymphocyte transformation and determine the levels of serum interleukin(IL)-2 and interferon-gamma(IFN-γ) and immune function by different compatibility of emblica total phenolic acids and flavonoids ig 9 days.Result: The compatibility of different proportions of total phenolic flavonoids emblica significantly inhibited the liver tumor cells,when the ratio of flavonoidsand the total acid was 10∶1(110 mg·L-1∶11 mg·L-1) inhibiting rate was the highest.The compatibility of total Flavonoids(228 mg) and total phenolic acids(329.5 mg) promoted the proliferation of mouse spleen lymphocytes,and stimulation index was higher than the control group,promoting secretion of lymphocytes factors.Same results were found in the compatibility of total flavonoids(4.56 mg)and total phenolic acids(6.59 mg).Conclusion: Emblica total phenolic acids and flavonoids significantly can inhibite cell proliferation and enhance the body’s specific immune function and non-specific immune function