HPLC测定11种中药口服液中6种防腐剂的含量

目的: 建立HPLC同时测定中药口服液体制剂中防腐剂含量的方法。方法: Kromasil C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.02 mol ·L^-1乙酸铵溶液梯度洗脱,流速1.0 mL ·min^-1,检测波长240 nm,柱温30 ℃。结果: 苯甲酸、山梨酸、对羟基苯甲酸酯类在1.0~181.1 mg ·L^-1线性关系良好(r>0.999);平均回收率95.5%~100.8%(RSD 0.7%~1.5%)。结论: 方法简单、可靠,精确,可用于中药口服液体制剂中防腐剂含量的测定。

Simultaneous Determination of Preservatives in Traditional Chinese Oral Liquid Preparation by HPLC

Objective: To establish a HPLC method for simultaneous determination of preservatives in traditional chinese oral liquid preparation.Method: The chromatographic separation was achieved on a Kromasil C18(4.6 mm×150 mm,5 μm) column with acetonitrile-0.02 mol·L-1 ammonium acetate solution as mobile phase(gradient elution).The flow rate was 1.0 mL·min-1,the detective wavelength was set at 240 nm,and the column temperature was maintained at 30 ℃.Result: Benzoic acid,sorbic acid and parahydroxybenzoate ester have a good resolution and linear correlation in certain range,respectively(r>0.999).The average recoverieswere 95.5%-100.8%(RSD 1.5%-1.8%).Conclusions: The method is simple,reliable and accurate for determination of preservatives in traditional chinese oral liquid preparation.