扶正解毒颗粒对染镍大鼠肾组织核因子-κB活化的影响

目的: 研究中药扶正解毒颗粒(FJG)对硫酸镍(NiSO₄)染毒大鼠肾组织核因子-κB (NF-κB)活化的影响。方法: 50只Wistar大鼠采用NiSO₄ 2.5 mg ·kg^-1 ip(连续7 d,此后间日1次)制备肾损伤模型,随机分为NiSO₄组(模型组)、 FJG高、中、低剂量组(分别为20,10,5 g ·kg^-1 ·d^-1),二硫基丁二酸(DMSA),50 mg ·kg^-1 ·d^-1)组。另设NS组(空白对照)及FJG对照组(FJG 10 g ·kg^-1 ·d^-1)。各组大鼠ig 1次/d,共4周。测定各组大鼠血尿素氮(BUN)、肌酐(SCr)及24 h尿蛋白定量(24 h-UP),免疫组化SP法观察肾组织NF-κB活化。结果: NiSO₄组大鼠出现肾功能损伤,NF-κB活化:肾小球(34.62±9.11)%,肾小管(62.45±14.78)%; FJG大剂量组NF-κB活化:肾小球(2.08±0.64)%,肾小管(11.48±3.39)%,明显低于NiSO₄组(P<0. 01),肾功能损伤亦明显减轻(P<0.05,P<0. 01)。结论: FJG可能通过抑制肾组织NF-κB活性的异常升高而发挥拮抗镍性肾损伤的作用。

Effects of Fuzheng Jiedu Granula on the Activation of NF-κB in Rats Exposed to Nickel

Objective:To study the effects of Fuzheng Jiedu Granula(FJG) on the activation of nuclear factor-kappa-B(NF-κB) in rats exposed to nickel(nickel sulfate, NiSO₄). Method: Rat nephrotoxicity model was established by intraperitoneal injection of NiSO₄ (2.5 mg ·kg^-1 ·d^-1).They were randomized into 5 groups,model group, FJG high dose group (20 g ·kg^-1 ·d^-1), FJG middle dose group(10 g ·kg^-1 ·d^-1), FJG,low dose group(5 g ·kg^-1 ·d^-1), meso-2,3-dimercaptosuccinic acid(DMSA) group(0.05 g ·kg^-1 ·d^-1). Twenty Wistar rats were randomly divided into NS group and FJG control group(10 g ·kg^-1 ·d^-1). Rats in each group were treated by intragastric administration once every day for four weeks. The levels of blood ureanitrogen(BUN),Creatinine(Scr) and 24 hours-proteinuria(24 h-UP) were measured.The activation of NF-κB was measured with immunohistochemistry methods. Result: In FJG groups,the levels of BUN,Scr,24h-UP and the activation of NF-κB were all decreased(P<0.05, P<0.01), compared with that of NiSO₄ group. Conclusion: Inhibiting the activation of NF-κB,FJG could protect renal from injury induced by nickel.