玉屏风煎剂中氨基酸类成分HPLC指纹图谱分析

目的: 建立玉屏风煎剂中氨基酸类成分的HPLC指纹图谱分析方法,探讨不同配伍对氨基酸类指纹图谱的影响。方法: 采用2,4-二硝基氟苯柱前衍生法,以精氨酸为参照物。Kromasil C₁₈色谱柱(4.6 nm×250 nm, 5 μm),流动相A为乙腈,B为N,N-二甲基甲酰胺-0.025 mol ·L^-1醋酸钠(1∶100,用36%乙酸调pH 6.0),梯度系统,柱温40 ℃,检测波长为360 nm,流速1.0 mL ·min^-1,进样量10 μL,分析时间70 min。结果: 共标出18个共有峰,鉴定出15种对人体有益的氨基酸成分。除1批样品相似度为0.916外,9批样品相似度均大于0.98。对整方氨基酸指纹图谱的影响从大到小依次是黄芪、炒白术、防风。结论: 所建立的玉屏风煎剂中氨基酸类成分指纹图谱特征性强,可作为玉屏风煎剂质量控制的手段之一,并可为中药复方配伍化学成分变化研究提供一定参考。

Analysis on Fingerprint of Amino Acids Composition of Yupingfeng Decoction by HPLC

Objective: To establish a fingerprint analysis method for amino acids composition of Yupingfeng decoction by HPLC and explore its various combinations on amino acid composition.Method: According to pre-column derivatization method for 2,4-dinitrofluorobenzene,gradient elution with N,N-dimethylformamide-0.025 mol·L-1 sodium acetate(1∶100,with 36% acetic acid adjust pH 6.0)-acetonitrile at the flow rate of 1.0 mL·min-1 in a Kromasil C18(4.6 nm×250 mm,5 μm) Column.The temperature of column was at 40 ℃.The UV detection wave length was set at 360 nm.Incoming sample amount was for 10 μL,analysis of time was for 70 min.Result: Eighteen co-possessing peaks were selected as the fingerprint peaks,15 kinds of beneficial amino acid composition were identified.Except for the fingerprint similarity value of one batch was 0.916,the fingerprint similarity value of the other nine batches was above 0.98.The impact of amino acid fingerprint is in descending order Astragalus membranaceus,Fried Atractylodes macrocephala Saposhnikovia.Conclusion: The fingerprint is characteristic,can be used for the quality control of Yupingfeng decoction,and can provide a reference for the research of the changes of chemical composition of traditional Chinese medicine compatibility.