葡萄籽原花青素对犬肝动脉栓塞性肝损伤的保护作用

目的观察葡萄籽原花青素（GSPE）对犬碘化油栓塞性肝损伤的保护作用。方法健康家犬12只,随机分为3组：A组经导管向肝动脉注入碘化油10ml;B组经导管向肝动脉注入碘化油10ml＋GSPE饱和溶液5ml;C组经导管向肝动脉注入生理盐水10ml。测定各组犬血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）活性和丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）的含量,同时对肝组织进行病理学检查。结果栓塞后A、B组犬血清ALT、AST值、MDA和TNF-α含量明显高于C组（P均〈0.05）,肝组织的病理损伤明显;栓塞后B组犬血清ALT、AST值、MDA和TNF-α含量明显低于A组（P均〈0.05）,且肝组织病理损伤程度较A组减轻。结论碘化油栓塞犬肝动脉可成功建立犬急性肝损伤模型。GSPE对犬碘化油栓塞性肝损伤具有明显保护作用,其机制可能与其强大的抗氧化作用及抑制炎症反应有关。

Protective effects of grape seed proanthocyanidins extract on liver injury caused by transcatheter hepatic arterial embolization in dogs

Objective To explore the protective effects of grape seed proanthocyanidins extracts(GSPE) on liver injury caused by transcatheter hepatic arterial embolization with lipiodol.Methods Twelve healthy dogs were randomly divided into 3 groups,and transcatheter hepatic arterial embolization was performed.In group A,10 ml lipiodol was infused into hepatic artery,while in group B,10 ml lipiodol and 5 ml GSPE saturated solution were infused.Dogs in group C received 10 ml saline.The blood serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),malondialdehyde(MDA) and tumor necrosis factor-α(TNF-α) were analyzed and compared statistically.Pathological changes of the liver were also observed.Results After embolization,the serum levels of ALT,AST,MDA and TNF-α in group A and group B were significantly highter than those in group C(all P<0.05),as well as the injury of liver tissue;while the serum indicators in group B were obviously lower than those in group A(all P<0.05),and also the damage of liver tissue.Conclusion Dogs’ liver injury was successfully induced by transcatheter hepatic arterial embolization with lipiodol.GSPE can effectively prevent liver injury induced by transcatheter hepatic arterial embolization with lipiodol.The mechanism probably relate to the antioxidant and anti-inflammatory activities of GSPE.